Conformation-dependent single-chain variable fragment antibodies specifically recognize beta-amyloid oligomers

Xiao ping Wang, Jun hua Zhang, Yu jiong Wang, Ying Feng, Xi Zhang, Xiao xia Sun, Ji liang Li, Xue ting Du, Mary P. Lambert, Shi gao Yang, Min Zhao, William L. Klein, Rui tian Liu*

*Corresponding author for this work

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

Increasing evidence indicates that beta-amyloid (Aβ) oligomers rather than monomers or fibrils are the major toxic agents that specifically inhibit synaptic plasticity and long-term potentiation (LTP) in Alzheimer's disease (AD). Neutralization of Aβ oligomeric toxicity was found to reverse memory deficits. Here, we report four single-chain variable fragment (scFv) antibodies isolated from the naive human scFv library by phage display that specifically recognized Aβ oligomers but not monomers and fibrils. These conformation-dependent scFv antibodies inhibit both Aβ fibrillation and cytotoxicity and bind to the same type of eptitope displayed on the Aβ oligomers. Such scFv antibodies specifically targeting toxic Aβ oligomers may have potential therapeutic and diagnostic applications for AD.

Original languageEnglish (US)
Pages (from-to)579-584
Number of pages6
JournalFEBS Letters
Volume583
Issue number3
DOIs
StatePublished - Feb 4 2009

Keywords

  • Alzheimer's disease
  • Beta-amyloid
  • Epitope
  • Oligomer
  • Single-chain variable fragment

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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