Abstract
Enhanced biological phosphorus removal (EBPR) is strongly influenced by the influent ratio of readily biodegradable carbon to soluble phosphorus due to the preferences of phosphorus-accumulating organisms (PAO). The sidestream EBPR (S2EBPR) process redirects a portion of return activated sludge (RAS) to a sidestream fermenter, increasing the availability of biodegradable carbon. In this study, we assessed the performance and microbial community structure of a full-scale S2EBPR demonstration supplemented with external carbon dosing. By the end of the 10 month study period, the demonstration achieved a median effluent orthophosphate of 0.3 mg/L. The microbial community consisted of a common core microbiome in the RAS fermenter, EBPR basin, and nitrification basin. The most abundant PAO detected were Ca. “Dechloromonas phosphorivorans”, while the canonical PAO Ca. “Accumulibacter” and Tetrasphaera were observed in lower relative abundance. In addition to non-canonical PAO enrichment, the glycogen-accumulating organism Ca. “Competibacter” proliferated throughout the study and at some points outnumbered PAO taxa by 30 to 1 with no tangible performance impacts. This study provides insights into successful S2EBPR implementation at a low-carbon facility and improves our understanding of microbial community structure and key PAO and GAO in S2EBPR systems.
Original language | English (US) |
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Pages (from-to) | 2384-2394 |
Number of pages | 11 |
Journal | ACS ES and T Water |
Volume | 3 |
Issue number | 8 |
DOIs | |
State | Published - Aug 11 2023 |
Funding
This work was supported by the Israel-U.S. Collaborative Water-Energy Research Center (CoWERC) via the Binational Industrial Research and Development Foundation (BIRD) Energy Center grant EC-15. This work was also supported in part through the computational resources and staff contributions provided for the Quest high performance computing facility at Northwestern University, which is jointly supported by the Office of the Provost, the Office for Research, and Northwestern University Information Technology.
Keywords
- GAO
- PAO
- RAS fermentation
- S2EBPR
- phosphorus
ASJC Scopus subject areas
- Chemistry (miscellaneous)
- Chemical Engineering (miscellaneous)
- Environmental Chemistry
- Water Science and Technology