Abstract
Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.
Original language | English (US) |
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Pages (from-to) | 164-173 |
Number of pages | 10 |
Journal | Experimental eye research |
Volume | 171 |
DOIs | |
State | Published - Jun 2018 |
Funding
The authors thank Griff Samples, Thelma De Souza, Aerie Pharmaceuticals, Bausch and Lomb, Alcon/Novartis, Glaukos and the Western Glaucoma Foundation for continued support of the trabecular meshwork study club; enabling 16 annual scientific gatherings of researchers dedicated to better understanding trabecular meshwork biology and facilitating the assembly of this position paper.
Keywords
- Aqueous humor dynamics
- Conventional outflow
- Glaucoma
- Intraocular pressure
- Ocular hypertension
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience