Abstract
Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 164-173 |
| Number of pages | 10 |
| Journal | Experimental Eye Research |
| Volume | 171 |
| DOIs | |
| State | Published - Jun 1 2018 |
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Keywords
- Aqueous humor dynamics
- Conventional outflow
- Glaucoma
- Intraocular pressure
- Ocular hypertension
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience
Cite this
}
Consensus recommendations for trabecular meshwork cell isolation, characterization and culture. / Keller, Kate E.; Bhattacharya, Sanjoy K.; Borrás, Theresa; Brunner, Thomas M.; Chansangpetch, Sunee; Clark, Abbott F.; Dismuke, W. Michael; Du, Yiqin; Elliott, Michael H.; Ethier, C. Ross; Faralli, Jennifer A.; Freddo, Thomas F.; Fuchshofer, Rudolf; Giovingo, Michael; Gong, Haiyan; Gonzalez, Pedro; Huang, Alex; Johnstone, Murray A.; Kaufman, Paul L.; Kelley, Mary J.; Knepper, Paul A.; Kopczynski, Casey C.; Kuchtey, John G.; Kuchtey, Rachel W.; Kuehn, Markus H.; Lieberman, Raquel L.; Lin, Shan C.; Liton, Paloma; Liu, Yutao; Lütjen-Drecoll, Elke; Mao, Weiming; Masis-Solano, Marisse; McDonnell, Fiona; McDowell, Colleen M.; Overby, Darryl R.; Pattabiraman, Padmanabhan P.; Raghunathan, Vijay K.; Rao, P. Vasanth; Rhee, Douglas J.; Chowdhury, Uttio Roy; Russell, Paul; Samples, John R.; Schwartz, Donald; Stubbs, Evan B.; Tamm, Ernst R.; Tan, James C.; Toris, Carol B.; Torrejon, Karen Y.; Vranka, Janice A.; Wirtz, Mary K.; Yorio, Thomas; Zhang, Jie; Zode, Gulab S.; Fautsch, Michael P.; Peters, Donna M.; Acott, Ted S.; Stamer, W. Daniel.
In: Experimental Eye Research, Vol. 171, 01.06.2018, p. 164-173.Research output: Contribution to journal › Short survey
TY - JOUR
T1 - Consensus recommendations for trabecular meshwork cell isolation, characterization and culture
AU - Keller, Kate E.
AU - Bhattacharya, Sanjoy K.
AU - Borrás, Theresa
AU - Brunner, Thomas M.
AU - Chansangpetch, Sunee
AU - Clark, Abbott F.
AU - Dismuke, W. Michael
AU - Du, Yiqin
AU - Elliott, Michael H.
AU - Ethier, C. Ross
AU - Faralli, Jennifer A.
AU - Freddo, Thomas F.
AU - Fuchshofer, Rudolf
AU - Giovingo, Michael
AU - Gong, Haiyan
AU - Gonzalez, Pedro
AU - Huang, Alex
AU - Johnstone, Murray A.
AU - Kaufman, Paul L.
AU - Kelley, Mary J.
AU - Knepper, Paul A.
AU - Kopczynski, Casey C.
AU - Kuchtey, John G.
AU - Kuchtey, Rachel W.
AU - Kuehn, Markus H.
AU - Lieberman, Raquel L.
AU - Lin, Shan C.
AU - Liton, Paloma
AU - Liu, Yutao
AU - Lütjen-Drecoll, Elke
AU - Mao, Weiming
AU - Masis-Solano, Marisse
AU - McDonnell, Fiona
AU - McDowell, Colleen M.
AU - Overby, Darryl R.
AU - Pattabiraman, Padmanabhan P.
AU - Raghunathan, Vijay K.
AU - Rao, P. Vasanth
AU - Rhee, Douglas J.
AU - Chowdhury, Uttio Roy
AU - Russell, Paul
AU - Samples, John R.
AU - Schwartz, Donald
AU - Stubbs, Evan B.
AU - Tamm, Ernst R.
AU - Tan, James C.
AU - Toris, Carol B.
AU - Torrejon, Karen Y.
AU - Vranka, Janice A.
AU - Wirtz, Mary K.
AU - Yorio, Thomas
AU - Zhang, Jie
AU - Zode, Gulab S.
AU - Fautsch, Michael P.
AU - Peters, Donna M.
AU - Acott, Ted S.
AU - Stamer, W. Daniel
PY - 2018/6/1
Y1 - 2018/6/1
N2 - Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.
AB - Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.
KW - Aqueous humor dynamics
KW - Conventional outflow
KW - Glaucoma
KW - Intraocular pressure
KW - Ocular hypertension
UR - http://www.scopus.com/inward/record.url?scp=85045559921&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85045559921&partnerID=8YFLogxK
U2 - 10.1016/j.exer.2018.03.001
DO - 10.1016/j.exer.2018.03.001
M3 - Short survey
VL - 171
SP - 164
EP - 173
JO - Experimental Eye Research
JF - Experimental Eye Research
SN - 0014-4835
ER -