Consensus recommendations for trabecular meshwork cell isolation, characterization and culture

Kate E. Keller; Sanjoy K. Bhattacharya; Theresa Borrás; Thomas M. Brunner; Sunee Chansangpetch; Abbott F. Clark; W. Michael Dismuke; Yiqin Du; Michael H. Elliott; C. Ross Ethier; Jennifer A. Faralli; Thomas F. Freddo; Rudolf Fuchshofer; Michael Giovingo; Haiyan Gong; Pedro Gonzalez; Alex Huang; Murray A. Johnstone; Paul L. Kaufman; Mary J. Kelley; Paul A. Knepper; Casey C. Kopczynski; John G. Kuchtey; Rachel W. Kuchtey; Markus H. Kuehn; Raquel L. Lieberman; Shan C. Lin; Paloma Liton; Yutao Liu; Elke Lütjen-Drecoll; Weiming Mao; Marisse Masis-Solano; Fiona McDonnell; Colleen M. McDowell; Darryl R. Overby; Padmanabhan P. Pattabiraman; Vijay K. Raghunathan; P. Vasanth Rao; Douglas J. Rhee; Uttio Roy Chowdhury; Paul Russell; John R. Samples; Donald Schwartz; Evan B. Stubbs; Ernst R. Tamm; James C. Tan; Carol B. Toris; Karen Y. Torrejon; Janice A. Vranka; Mary K. Wirtz; Thomas Yorio; Jie Zhang; Gulab S. Zode; Michael P. Fautsch; Donna M. Peters; Ted S. Acott; W. Daniel Stamer

doi:10.1016/j.exer.2018.03.001

Consensus recommendations for trabecular meshwork cell isolation, characterization and culture

Kate E. Keller, Sanjoy K. Bhattacharya, Theresa Borrás, Thomas M. Brunner, Sunee Chansangpetch, Abbott F. Clark, W. Michael Dismuke, Yiqin Du, Michael H. Elliott, C. Ross Ethier, Jennifer A. Faralli, Thomas F. Freddo, Rudolf Fuchshofer, Michael Giovingo, Haiyan Gong, Pedro Gonzalez, Alex Huang, Murray A. Johnstone, Paul L. Kaufman, Mary J. KelleyPaul A. Knepper, Casey C. Kopczynski, John G. Kuchtey, Rachel W. Kuchtey, Markus H. Kuehn, Raquel L. Lieberman, Shan C. Lin, Paloma Liton, Yutao Liu, Elke Lütjen-Drecoll, Weiming Mao, Marisse Masis-Solano, Fiona McDonnell, Colleen M. McDowell, Darryl R. Overby, Padmanabhan P. Pattabiraman, Vijay K. Raghunathan, P. Vasanth Rao, Douglas J. Rhee, Uttio Roy Chowdhury, Paul Russell, John R. Samples, Donald Schwartz, Evan B. Stubbs, Ernst R. Tamm, James C. Tan, Carol B. Toris, Karen Y. Torrejon, Janice A. Vranka, Mary K. Wirtz, Thomas Yorio, Jie Zhang, Gulab S. Zode, Michael P. Fautsch, Donna M. Peters, Ted S. Acott, W. Daniel Stamer^*

^*Corresponding author for this work

Ophthalmology

Research output: Contribution to journal › Short survey › peer-review

91 Scopus citations

Abstract

Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.

Original language	English (US)
Pages (from-to)	164-173
Number of pages	10
Journal	Experimental eye research
Volume	171
DOIs	https://doi.org/10.1016/j.exer.2018.03.001
State	Published - Jun 2018

Keywords

Aqueous humor dynamics
Conventional outflow
Glaucoma
Intraocular pressure
Ocular hypertension

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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10.1016/j.exer.2018.03.001

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Keller, K. E., Bhattacharya, S. K., Borrás, T., Brunner, T. M., Chansangpetch, S., Clark, A. F., Dismuke, W. M., Du, Y., Elliott, M. H., Ethier, C. R., Faralli, J. A., Freddo, T. F., Fuchshofer, R., Giovingo, M., Gong, H., Gonzalez, P., Huang, A., Johnstone, M. A., Kaufman, P. L., ... Stamer, W. D. (2018). Consensus recommendations for trabecular meshwork cell isolation, characterization and culture. Experimental eye research, 171, 164-173. https://doi.org/10.1016/j.exer.2018.03.001

Keller, Kate E. ; Bhattacharya, Sanjoy K. ; Borrás, Theresa ; Brunner, Thomas M. ; Chansangpetch, Sunee ; Clark, Abbott F. ; Dismuke, W. Michael ; Du, Yiqin ; Elliott, Michael H. ; Ethier, C. Ross ; Faralli, Jennifer A. ; Freddo, Thomas F. ; Fuchshofer, Rudolf ; Giovingo, Michael ; Gong, Haiyan ; Gonzalez, Pedro ; Huang, Alex ; Johnstone, Murray A. ; Kaufman, Paul L. ; Kelley, Mary J. ; Knepper, Paul A. ; Kopczynski, Casey C. ; Kuchtey, John G. ; Kuchtey, Rachel W. ; Kuehn, Markus H. ; Lieberman, Raquel L. ; Lin, Shan C. ; Liton, Paloma ; Liu, Yutao ; Lütjen-Drecoll, Elke ; Mao, Weiming ; Masis-Solano, Marisse ; McDonnell, Fiona ; McDowell, Colleen M. ; Overby, Darryl R. ; Pattabiraman, Padmanabhan P. ; Raghunathan, Vijay K. ; Rao, P. Vasanth ; Rhee, Douglas J. ; Chowdhury, Uttio Roy ; Russell, Paul ; Samples, John R. ; Schwartz, Donald ; Stubbs, Evan B. ; Tamm, Ernst R. ; Tan, James C. ; Toris, Carol B. ; Torrejon, Karen Y. ; Vranka, Janice A. ; Wirtz, Mary K. ; Yorio, Thomas ; Zhang, Jie ; Zode, Gulab S. ; Fautsch, Michael P. ; Peters, Donna M. ; Acott, Ted S. ; Stamer, W. Daniel. / Consensus recommendations for trabecular meshwork cell isolation, characterization and culture. In: Experimental eye research. 2018 ; Vol. 171. pp. 164-173.

@article{2a9edc5d1a744505ab16e9e0aa03ae06,

title = "Consensus recommendations for trabecular meshwork cell isolation, characterization and culture",

abstract = "Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.",

keywords = "Aqueous humor dynamics, Conventional outflow, Glaucoma, Intraocular pressure, Ocular hypertension",

author = "Keller, {Kate E.} and Bhattacharya, {Sanjoy K.} and Theresa Borr{\'a}s and Brunner, {Thomas M.} and Sunee Chansangpetch and Clark, {Abbott F.} and Dismuke, {W. Michael} and Yiqin Du and Elliott, {Michael H.} and Ethier, {C. Ross} and Faralli, {Jennifer A.} and Freddo, {Thomas F.} and Rudolf Fuchshofer and Michael Giovingo and Haiyan Gong and Pedro Gonzalez and Alex Huang and Johnstone, {Murray A.} and Kaufman, {Paul L.} and Kelley, {Mary J.} and Knepper, {Paul A.} and Kopczynski, {Casey C.} and Kuchtey, {John G.} and Kuchtey, {Rachel W.} and Kuehn, {Markus H.} and Lieberman, {Raquel L.} and Lin, {Shan C.} and Paloma Liton and Yutao Liu and Elke L{\"u}tjen-Drecoll and Weiming Mao and Marisse Masis-Solano and Fiona McDonnell and McDowell, {Colleen M.} and Overby, {Darryl R.} and Pattabiraman, {Padmanabhan P.} and Raghunathan, {Vijay K.} and Rao, {P. Vasanth} and Rhee, {Douglas J.} and Chowdhury, {Uttio Roy} and Paul Russell and Samples, {John R.} and Donald Schwartz and Stubbs, {Evan B.} and Tamm, {Ernst R.} and Tan, {James C.} and Toris, {Carol B.} and Torrejon, {Karen Y.} and Vranka, {Janice A.} and Wirtz, {Mary K.} and Thomas Yorio and Jie Zhang and Zode, {Gulab S.} and Fautsch, {Michael P.} and Peters, {Donna M.} and Acott, {Ted S.} and Stamer, {W. Daniel}",

note = "Funding Information: The authors thank Griff Samples, Thelma De Souza, Aerie Pharmaceuticals, Bausch and Lomb, Alcon/Novartis, Glaukos and the Western Glaucoma Foundation for continued support of the trabecular meshwork study club; enabling 16 annual scientific gatherings of researchers dedicated to better understanding trabecular meshwork biology and facilitating the assembly of this position paper. Publisher Copyright: {\textcopyright} 2018",

year = "2018",

month = jun,

doi = "10.1016/j.exer.2018.03.001",

language = "English (US)",

volume = "171",

pages = "164--173",

journal = "Experimental Eye Research",

issn = "0014-4835",

publisher = "Academic Press Inc.",

}

Keller, KE, Bhattacharya, SK, Borrás, T, Brunner, TM, Chansangpetch, S, Clark, AF, Dismuke, WM, Du, Y, Elliott, MH, Ethier, CR, Faralli, JA, Freddo, TF, Fuchshofer, R, Giovingo, M, Gong, H, Gonzalez, P, Huang, A, Johnstone, MA, Kaufman, PL, Kelley, MJ, Knepper, PA, Kopczynski, CC, Kuchtey, JG, Kuchtey, RW, Kuehn, MH, Lieberman, RL, Lin, SC, Liton, P, Liu, Y, Lütjen-Drecoll, E, Mao, W, Masis-Solano, M, McDonnell, F, McDowell, CM, Overby, DR, Pattabiraman, PP, Raghunathan, VK, Rao, PV, Rhee, DJ, Chowdhury, UR, Russell, P, Samples, JR, Schwartz, D, Stubbs, EB, Tamm, ER, Tan, JC, Toris, CB, Torrejon, KY, Vranka, JA, Wirtz, MK, Yorio, T, Zhang, J, Zode, GS, Fautsch, MP, Peters, DM, Acott, TS & Stamer, WD 2018, 'Consensus recommendations for trabecular meshwork cell isolation, characterization and culture', Experimental eye research, vol. 171, pp. 164-173. https://doi.org/10.1016/j.exer.2018.03.001

Consensus recommendations for trabecular meshwork cell isolation, characterization and culture. / Keller, Kate E.; Bhattacharya, Sanjoy K.; Borrás, Theresa; Brunner, Thomas M.; Chansangpetch, Sunee; Clark, Abbott F.; Dismuke, W. Michael; Du, Yiqin; Elliott, Michael H.; Ethier, C. Ross; Faralli, Jennifer A.; Freddo, Thomas F.; Fuchshofer, Rudolf; Giovingo, Michael; Gong, Haiyan; Gonzalez, Pedro; Huang, Alex; Johnstone, Murray A.; Kaufman, Paul L.; Kelley, Mary J.; Knepper, Paul A.; Kopczynski, Casey C.; Kuchtey, John G.; Kuchtey, Rachel W.; Kuehn, Markus H.; Lieberman, Raquel L.; Lin, Shan C.; Liton, Paloma; Liu, Yutao; Lütjen-Drecoll, Elke; Mao, Weiming; Masis-Solano, Marisse; McDonnell, Fiona; McDowell, Colleen M.; Overby, Darryl R.; Pattabiraman, Padmanabhan P.; Raghunathan, Vijay K.; Rao, P. Vasanth; Rhee, Douglas J.; Chowdhury, Uttio Roy; Russell, Paul; Samples, John R.; Schwartz, Donald; Stubbs, Evan B.; Tamm, Ernst R.; Tan, James C.; Toris, Carol B.; Torrejon, Karen Y.; Vranka, Janice A.; Wirtz, Mary K.; Yorio, Thomas; Zhang, Jie; Zode, Gulab S.; Fautsch, Michael P.; Peters, Donna M.; Acott, Ted S.; Stamer, W. Daniel.

In: Experimental eye research, Vol. 171, 06.2018, p. 164-173.

Research output: Contribution to journal › Short survey › peer-review

TY - JOUR

T1 - Consensus recommendations for trabecular meshwork cell isolation, characterization and culture

AU - Keller, Kate E.

AU - Bhattacharya, Sanjoy K.

AU - Borrás, Theresa

AU - Brunner, Thomas M.

AU - Chansangpetch, Sunee

AU - Clark, Abbott F.

AU - Dismuke, W. Michael

AU - Du, Yiqin

AU - Elliott, Michael H.

AU - Ethier, C. Ross

AU - Faralli, Jennifer A.

AU - Freddo, Thomas F.

AU - Fuchshofer, Rudolf

AU - Giovingo, Michael

AU - Gong, Haiyan

AU - Gonzalez, Pedro

AU - Huang, Alex

AU - Johnstone, Murray A.

AU - Kaufman, Paul L.

AU - Kelley, Mary J.

AU - Knepper, Paul A.

AU - Kopczynski, Casey C.

AU - Kuchtey, John G.

AU - Kuchtey, Rachel W.

AU - Kuehn, Markus H.

AU - Lieberman, Raquel L.

AU - Lin, Shan C.

AU - Liton, Paloma

AU - Liu, Yutao

AU - Lütjen-Drecoll, Elke

AU - Mao, Weiming

AU - Masis-Solano, Marisse

AU - McDonnell, Fiona

AU - McDowell, Colleen M.

AU - Overby, Darryl R.

AU - Pattabiraman, Padmanabhan P.

AU - Raghunathan, Vijay K.

AU - Rao, P. Vasanth

AU - Rhee, Douglas J.

AU - Chowdhury, Uttio Roy

AU - Russell, Paul

AU - Samples, John R.

AU - Schwartz, Donald

AU - Stubbs, Evan B.

AU - Tamm, Ernst R.

AU - Tan, James C.

AU - Toris, Carol B.

AU - Torrejon, Karen Y.

AU - Vranka, Janice A.

AU - Wirtz, Mary K.

AU - Yorio, Thomas

AU - Zhang, Jie

AU - Zode, Gulab S.

AU - Fautsch, Michael P.

AU - Peters, Donna M.

AU - Acott, Ted S.

AU - Stamer, W. Daniel

N1 - Funding Information: The authors thank Griff Samples, Thelma De Souza, Aerie Pharmaceuticals, Bausch and Lomb, Alcon/Novartis, Glaukos and the Western Glaucoma Foundation for continued support of the trabecular meshwork study club; enabling 16 annual scientific gatherings of researchers dedicated to better understanding trabecular meshwork biology and facilitating the assembly of this position paper. Publisher Copyright: © 2018

PY - 2018/6

Y1 - 2018/6

N2 - Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.

AB - Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.

KW - Aqueous humor dynamics

KW - Conventional outflow

KW - Glaucoma

KW - Intraocular pressure

KW - Ocular hypertension

UR - http://www.scopus.com/inward/record.url?scp=85045559921&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85045559921&partnerID=8YFLogxK

U2 - 10.1016/j.exer.2018.03.001

DO - 10.1016/j.exer.2018.03.001

M3 - Short survey

C2 - 29526795

AN - SCOPUS:85045559921

VL - 171

SP - 164

EP - 173

JO - Experimental Eye Research

JF - Experimental Eye Research

SN - 0014-4835

ER -

Consensus recommendations for trabecular meshwork cell isolation, characterization and culture

Abstract

Keywords

ASJC Scopus subject areas

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