Considerations for MALDI-Based Quantitative Mass Spectrometry Imaging Studies

Fernando Tobias*, Amanda B. Hummon

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

27 Scopus citations


Significant advances in mass spectrometry imaging (MSI) have pushed the boundaries in obtaining spatial information and quantification in biological samples. Quantitative MSI (qMSI) has typically been challenging to achieve because of matrix and tissue heterogeneity, inefficient analyte extraction, and ion suppression effects, but recent studies have demonstrated approaches to obtain highly robust methods and reproducible results. In this perspective, we share our insights into sample preparation, how the choice of matrix influences sensitivity, construction of calibration curves, signal normalization, and visualization of MSI data. We hope that by articulating these guidelines that qMSI can be routinely conducted while retaining the analytical merits of other mass spectrometry modalities.

Original languageEnglish (US)
Pages (from-to)3620-3630
Number of pages11
JournalJournal of Proteome Research
Issue number9
StatePublished - Sep 4 2020
Externally publishedYes


  • calibration curve
  • color schemes
  • mass spectrometry imaging
  • matrix
  • quantitative

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry


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