Abstract
Fermenting glucose in the presence of enough oxygen to support respiration, known as aerobic glycolysis, is believed to maximize growth rate. We observed increasing aerobic glycolysis during exponential growth, suggesting additional physiological roles for aerobic glycolysis. We investigated such roles in yeast batch cultures by quantifying O2 consumption, CO2 production, amino acids, mRNAs, proteins, posttranslational modifications, and stress sensitivity in the course of nine doublings at constant rate. During this course, the cells support a constant biomass-production rate with decreasing rates of respiration and ATP production but also decrease their stress resistance. As the respiration rate decreases, so do the levels of enzymes catalyzing rate-determining reactions of the tricarboxylic-acid cycle (providing NADH for respiration) and of mitochondrial folate-mediated NADPH production (required for oxidative defense). The findings demonstrate that exponential growth can represent not a single metabolic/physiological state but a continuum of changing states and that aerobic glycolysis can reduce the energy demands associated with respiratory metabolism and stress survival.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 705-714 |
| Number of pages | 10 |
| Journal | Cell reports |
| Volume | 7 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2014 |
Funding
We thank S. Manalis for the use of his Coulter counter and K. Chatman for assistance with measuring amino acid levels, as well as J. Rabinowitz, D. Botstein, N. Wingreen, A. Murray, Q. Justman, E. Solis, and members of the van Oudenaarden lab for critical discussions. This work was funded by grants from the NIH to A.v.O. (DP1 CA174420, R01-GM068957, and U54CA143874) and E.M.A. (R01-GM-096193) and an Alfred P. Sloan Research Fellowship to E.M.A.
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology