Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein

M. Milivojevic, I. Petrovic*, N. Kovacevic-Grujicic, J. Popovic, M. Mojsin, M. Stevanovic

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

SOX18 transcription factor plays important roles in a range of biological processes such as vasculogenesis, hair follicle development, lymphangiogenesis, atherosclerosis, and angiogenesis. In this paper we present the generation of a novel SOX18 dominant-negative mutant (SOX18DN) encoding truncated SOX18 protein that lacks a trans-activation domain. We show that both wild-type SOX18 (SOX18wt) and truncated human SOX18 proteins are able to bind to their consensus sequence in vitro. Functional analysis confirmed that SOX18wt has potent trans-activation properties, while SOX18DN displays dominant-negative effect. We believe that these SOX18wt and SOX18DN expression constructs could be successfully used for further characterization of the function of this protein.

Original languageEnglish (US)
Pages (from-to)1287-1292
Number of pages6
JournalBiochemistry (Moscow)
Volume78
Issue number11
DOIs
StatePublished - Nov 2013

Keywords

  • dominant-negative
  • SOX18
  • transcription factor
  • truncated protein

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Geriatrics and Gerontology

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