Construction of a Neisseria gonorrhoeae MS11 derivative deficient in NgoMI restriction and modification

D. C. Stein*, R. Chien, H. S. Seifert

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

We have cloned from Neisseria gonorrhoeae MS11 the gene encoding a methylase that modifies the sequence GCCGGC. The corresponding restriction enzyme was also encoded by this clone. Sequence analysis demonstrated that the methylase shares sequence similarities with other cytosine methylases, but the sequence organization of M.NgoMI is different from that seen for other cytosine methylases. A deletion was introduced into the chromosome of N. gonorrhoeae MS11 to produce strain MUG701, a strain that is inactivated in both the methylase and the restriction genes. Although this strain no longer methylated its DNA at the NgoMI recognition sequence, cells were viable and had no other significant phenotypic changes. Transformation data indicated that MS11 does not produce enough restriction activity to block plasmid transformation in the gonococcus, even though restriction activity could be demonstrated in E. coli containing the cloned gene.

Original languageEnglish (US)
Pages (from-to)4899-4906
Number of pages8
JournalJournal of bacteriology
Volume174
Issue number15
DOIs
StatePublished - 1992

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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