A PCR-based screening approach was used to isolate six yeast artificial chromosome (YAC) clones containing segments of the human α5(IV) collagen gene (COL4A5). This gene is located at Xq22 and is known to be involved in the kidney disorder known as Alport syndrome (AS). By analyzing sequence-tagged sites, cDNA content, and rare-cutting restriction site patterns in these YAC clones, a contig that spans the entirety of the α5(IV) gene was constructed. This contig may contain as much as 690 kb of DNA from the α5(IV) locus. On the basis of the information obtained from these YAC clones, the genomic map and gene structure of the α5(IV) gene have been refined. This study has also provided a valuable resource for subsequent studies of the α5(IV) gene and its flanking DNA sequences.
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