Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes

Susan E H West; Mary Jo M Romero; Laura B. Regassa; Nicolette A. Zielinski; Rodney A. Welch

doi:10.1016/0378-1119(95)00236-Y

Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes

Susan E H West^*, Mary Jo M Romero, Laura B. Regassa, Nicolette A. Zielinski, Rodney A. Welch

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

We constructed several cloning vectors, designated pGZRS-18/19 and pGZRS-38/39, which were based on an endogenous Actinobacillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the lacZα-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative Apl promoter or the Km^R gene from Tn903. These vectors replicate in representative strains of Apl serotypes 1 and 7, Escherichia coli, Pasteurella haemolytica (Ph) and Haemophilus (Actinobacillus) actinomycetemcomitans. We also found that Apl and Ph did not express genes under the control of the lacZ or bla promoters, suggesting that their RNA polymerases may not utilize these promoters.

Original language	English (US)
Pages (from-to)	81-86
Number of pages	6
Journal	Gene
Volume	160
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(95)00236-Y
State	Published - Jul 4 1995

Keywords

Pasteurella haemolytica
cloning
gene expression

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(95)00236-Y

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title = "Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes",

abstract = "We constructed several cloning vectors, designated pGZRS-18/19 and pGZRS-38/39, which were based on an endogenous Actinobacillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the lacZα-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative Apl promoter or the KmR gene from Tn903. These vectors replicate in representative strains of Apl serotypes 1 and 7, Escherichia coli, Pasteurella haemolytica (Ph) and Haemophilus (Actinobacillus) actinomycetemcomitans. We also found that Apl and Ph did not express genes under the control of the lacZ or bla promoters, suggesting that their RNA polymerases may not utilize these promoters.",

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T2 - expression of antibiotic-resistance genes

AU - West, Susan E H

AU - Romero, Mary Jo M

AU - Regassa, Laura B.

AU - Zielinski, Nicolette A.

AU - Welch, Rodney A.

PY - 1995/7/4

Y1 - 1995/7/4

N2 - We constructed several cloning vectors, designated pGZRS-18/19 and pGZRS-38/39, which were based on an endogenous Actinobacillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the lacZα-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative Apl promoter or the KmR gene from Tn903. These vectors replicate in representative strains of Apl serotypes 1 and 7, Escherichia coli, Pasteurella haemolytica (Ph) and Haemophilus (Actinobacillus) actinomycetemcomitans. We also found that Apl and Ph did not express genes under the control of the lacZ or bla promoters, suggesting that their RNA polymerases may not utilize these promoters.

AB - We constructed several cloning vectors, designated pGZRS-18/19 and pGZRS-38/39, which were based on an endogenous Actinobacillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the lacZα-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative Apl promoter or the KmR gene from Tn903. These vectors replicate in representative strains of Apl serotypes 1 and 7, Escherichia coli, Pasteurella haemolytica (Ph) and Haemophilus (Actinobacillus) actinomycetemcomitans. We also found that Apl and Ph did not express genes under the control of the lacZ or bla promoters, suggesting that their RNA polymerases may not utilize these promoters.

KW - Pasteurella haemolytica

KW - cloning

KW - gene expression

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