Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes

Susan E H West; Mary Jo M Romero; Laura B. Regassa; Nicolette A. Zielinski; Rodney A. Welch

doi:10.1016/0378-1119(95)00236-Y

Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes

Susan E H West^*, Mary Jo M Romero, Laura B. Regassa, Nicolette A. Zielinski, Rodney A. Welch

^*Corresponding author for this work

Center for Comparative Medicine

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

We constructed several cloning vectors, designated pGZRS-18/19 and pGZRS-38/39, which were based on an endogenous Actinobacillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the lacZα-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative Apl promoter or the Km^R gene from Tn903. These vectors replicate in representative strains of Apl serotypes 1 and 7, Escherichia coli, Pasteurella haemolytica (Ph) and Haemophilus (Actinobacillus) actinomycetemcomitans. We also found that Apl and Ph did not express genes under the control of the lacZ or bla promoters, suggesting that their RNA polymerases may not utilize these promoters.

Original language	English (US)
Pages (from-to)	81-86
Number of pages	6
Journal	Gene
Volume	160
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(95)00236-Y
State	Published - Jul 4 1995

Funding

This research was supported by funds from the School of Veterinary Medicine, University of Wisconsin-Madison (Madison, WI, USA) and by USDA competitive grant 91-37204-6562 awarded to S.E.H.W. and by USDA competitive grant 91-37204-6435 awarded to R.A.W. We would like to thank Janet MacInnes for providing the Apl strains CM5 and CM5ANG1 and the Apl lacZg ene; Thomas J. Inzana for Apl strains 4074 and K17; Thomas R. Shryock for Apl strains F6J, F6L and EL312; Michael J. Heuther for Apl strain AF1085-7; Sarah K. Highlander for Ph strains SH011 and PHL101; Glynn H. Frank for Ph strain A30 and serotyping the Ph strains; and the Veterinary School at Michigan State University for Ph strains 918227 and 932733-2. We would also like to thank Allen K. Sample for construction of pMKT8 and Robert Kazmierczak for excellent technical assistance.

Keywords

Pasteurella haemolytica
cloning
gene expression

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(95)00236-Y

Cite this

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title = "Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes",

abstract = "We constructed several cloning vectors, designated pGZRS-18/19 and pGZRS-38/39, which were based on an endogenous Actinobacillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the lacZα-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative Apl promoter or the KmR gene from Tn903. These vectors replicate in representative strains of Apl serotypes 1 and 7, Escherichia coli, Pasteurella haemolytica (Ph) and Haemophilus (Actinobacillus) actinomycetemcomitans. We also found that Apl and Ph did not express genes under the control of the lacZ or bla promoters, suggesting that their RNA polymerases may not utilize these promoters.",

keywords = "Pasteurella haemolytica, cloning, gene expression",

author = "West, {Susan E H} and Romero, {Mary Jo M} and Regassa, {Laura B.} and Zielinski, {Nicolette A.} and Welch, {Rodney A.}",

note = "Funding Information: This research was supported by funds from the School of Veterinary Medicine, University of Wisconsin-Madison (Madison, WI, USA) and by USDA competitive grant 91-37204-6562 awarded to S.E.H.W. and by USDA competitive grant 91-37204-6435 awarded to R.A.W. We would like to thank Janet MacInnes for providing the Apl strains CM5 and CM5ANG1 and the Apl lacZg ene; Thomas J. Inzana for Apl strains 4074 and K17; Thomas R. Shryock for Apl strains F6J, F6L and EL312; Michael J. Heuther for Apl strain AF1085-7; Sarah K. Highlander for Ph strains SH011 and PHL101; Glynn H. Frank for Ph strain A30 and serotyping the Ph strains; and the Veterinary School at Michigan State University for Ph strains 918227 and 932733-2. We would also like to thank Allen K. Sample for construction of pMKT8 and Robert Kazmierczak for excellent technical assistance.",

year = "1995",

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doi = "10.1016/0378-1119(95)00236-Y",

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T1 - Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors

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AU - West, Susan E H

AU - Romero, Mary Jo M

AU - Regassa, Laura B.

AU - Zielinski, Nicolette A.

AU - Welch, Rodney A.

N1 - Funding Information: This research was supported by funds from the School of Veterinary Medicine, University of Wisconsin-Madison (Madison, WI, USA) and by USDA competitive grant 91-37204-6562 awarded to S.E.H.W. and by USDA competitive grant 91-37204-6435 awarded to R.A.W. We would like to thank Janet MacInnes for providing the Apl strains CM5 and CM5ANG1 and the Apl lacZg ene; Thomas J. Inzana for Apl strains 4074 and K17; Thomas R. Shryock for Apl strains F6J, F6L and EL312; Michael J. Heuther for Apl strain AF1085-7; Sarah K. Highlander for Ph strains SH011 and PHL101; Glynn H. Frank for Ph strain A30 and serotyping the Ph strains; and the Veterinary School at Michigan State University for Ph strains 918227 and 932733-2. We would also like to thank Allen K. Sample for construction of pMKT8 and Robert Kazmierczak for excellent technical assistance.

PY - 1995/7/4

Y1 - 1995/7/4

N2 - We constructed several cloning vectors, designated pGZRS-18/19 and pGZRS-38/39, which were based on an endogenous Actinobacillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the lacZα-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative Apl promoter or the KmR gene from Tn903. These vectors replicate in representative strains of Apl serotypes 1 and 7, Escherichia coli, Pasteurella haemolytica (Ph) and Haemophilus (Actinobacillus) actinomycetemcomitans. We also found that Apl and Ph did not express genes under the control of the lacZ or bla promoters, suggesting that their RNA polymerases may not utilize these promoters.

AB - We constructed several cloning vectors, designated pGZRS-18/19 and pGZRS-38/39, which were based on an endogenous Actinobacillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the lacZα-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative Apl promoter or the KmR gene from Tn903. These vectors replicate in representative strains of Apl serotypes 1 and 7, Escherichia coli, Pasteurella haemolytica (Ph) and Haemophilus (Actinobacillus) actinomycetemcomitans. We also found that Apl and Ph did not express genes under the control of the lacZ or bla promoters, suggesting that their RNA polymerases may not utilize these promoters.

KW - Pasteurella haemolytica

KW - cloning

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Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes

Abstract

Funding

Keywords

ASJC Scopus subject areas

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