Continuous CD8 + T-cell priming by dendritic cell cross-presentation of persistent antigen following adeno-associated virus-mediated gene delivery

Dan Xu, Christopher M. Walker*

*Corresponding author for this work

Research output: Contribution to journalComment/debatepeer-review

12 Scopus citations

Abstract

Recombinant adeno-associated virus (rAAV) vectors establish persistent transgene expression in the skeletal muscle of mice. How dendritic cells acquire encoded antigens for CD8 + T-cell priming is unknown. Here we document CD8 + T-cell priming after lethal irradiation and bone marrow reconstitution of mice treated with an AAV vector several weeks earlier. Temporal separation of vector delivery and successful class I antigen presentation indicated that T-cell priming does not necessarily require antigen synthesis in AAV-transduced dendritic cells. An apparent cross-presentation of antigen acquired from muscle suggests that strategies to limit transgene expression in dendritic cells will not prevent unwanted CD8 + T-cell responses.

Original languageEnglish (US)
Pages (from-to)12083-12086
Number of pages4
JournalJournal of virology
Volume85
Issue number22
DOIs
StatePublished - Nov 2011

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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