Control of proliferin gene expression in serum-stimulated mouse cells.

D. I. Linzer*, E. L. Wilder

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The serum-inducible expression of proliferin genes in BALB/c 3T3 cells was found to be dependent on both protein synthesis and an extended presence of serum in the medium. Even though no mature proliferin mRNA was detected in serum-starved cells, transcription of the proliferin genes occurred in these resting-cell cultures, indicating that posttranscriptional events may be important for regulating proliferin mRNA levels. These results suggest that protein synthesis after serum stimulation of quiescent mouse fibroblasts is required for posttranscriptional processing or stabilization of proliferin RNA. Proliferin RNA levels were found to be heterogeneous among serum-stimulated cells analyzed by in situ hybridization. This heterogeneity is probably due to asynchrony in the population and may point to a correlation between the time of proliferin expression and the time of entry of a cell into S phase.

Original languageEnglish (US)
Pages (from-to)2080-2086
Number of pages7
JournalMolecular and cellular biology
Volume7
Issue number6
DOIs
StatePublished - Jun 1987

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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