Controlled Assembly of Artificial Protein-Protein Complexes via DNA Duplex Formation

Eliza Płoskoń; Sara C. Wagner; Andrew D. Ellington; Michael C. Jewett; Rachel O'Reilly; Paula J. Booth

doi:10.1021/bc500473s

Controlled Assembly of Artificial Protein-Protein Complexes via DNA Duplex Formation

Eliza Płoskoń^*, Sara C. Wagner, Andrew D. Ellington, Michael C. Jewett, Rachel O'Reilly, Paula J. Booth

^*Corresponding author for this work

Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

DNA-protein conjugates have found a wide range of applications. This study demonstrates the formation of defined, non-native protein-protein complexes via the site specific labeling of two proteins of interest with complementary strands of single-stranded DNA in vitro. This study demonstrates that the affinity of two DNA-protein conjugates for one another may be tuned by the use of variable lengths of DNA allowing reversible control of complex formation. (Figure Presented).

Original language	English (US)
Pages (from-to)	427-434
Number of pages	8
Journal	Bioconjugate Chemistry
Volume	26
Issue number	3
DOIs	https://doi.org/10.1021/bc500473s
State	Published - Mar 18 2015

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering
Pharmacology
Pharmaceutical Science
Organic Chemistry

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abstract = "DNA-protein conjugates have found a wide range of applications. This study demonstrates the formation of defined, non-native protein-protein complexes via the site specific labeling of two proteins of interest with complementary strands of single-stranded DNA in vitro. This study demonstrates that the affinity of two DNA-protein conjugates for one another may be tuned by the use of variable lengths of DNA allowing reversible control of complex formation. (Figure Presented).",

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AU - Płoskoń, Eliza

AU - Wagner, Sara C.

AU - Ellington, Andrew D.

AU - Jewett, Michael C.

AU - O'Reilly, Rachel

AU - Booth, Paula J.

PY - 2015/3/18

Y1 - 2015/3/18

N2 - DNA-protein conjugates have found a wide range of applications. This study demonstrates the formation of defined, non-native protein-protein complexes via the site specific labeling of two proteins of interest with complementary strands of single-stranded DNA in vitro. This study demonstrates that the affinity of two DNA-protein conjugates for one another may be tuned by the use of variable lengths of DNA allowing reversible control of complex formation. (Figure Presented).

AB - DNA-protein conjugates have found a wide range of applications. This study demonstrates the formation of defined, non-native protein-protein complexes via the site specific labeling of two proteins of interest with complementary strands of single-stranded DNA in vitro. This study demonstrates that the affinity of two DNA-protein conjugates for one another may be tuned by the use of variable lengths of DNA allowing reversible control of complex formation. (Figure Presented).

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Controlled Assembly of Artificial Protein-Protein Complexes via DNA Duplex Formation

Abstract

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