Controlled Assembly of Artificial Protein-Protein Complexes via DNA Duplex Formation

Eliza Płoskoń*, Sara C. Wagner, Andrew D. Ellington, Michael C. Jewett, Rachel O'Reilly, Paula J. Booth

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

DNA-protein conjugates have found a wide range of applications. This study demonstrates the formation of defined, non-native protein-protein complexes via the site specific labeling of two proteins of interest with complementary strands of single-stranded DNA in vitro. This study demonstrates that the affinity of two DNA-protein conjugates for one another may be tuned by the use of variable lengths of DNA allowing reversible control of complex formation. (Figure Presented).

Original languageEnglish (US)
Pages (from-to)427-434
Number of pages8
JournalBioconjugate Chemistry
Volume26
Issue number3
DOIs
StatePublished - Mar 18 2015

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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