Controlling Secretion in Artificial Cells with a Membrane and Gate

Claire E. Hilburger, Miranda L. Jacobs, Kamryn R. Lewis, Justin A. Peruzzi, Neha P. Kamat*

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The assembly of channel proteins into vesicle membranes is a useful strategy to control activities of vesicle-based systems. Here, we developed a membrane AND gate that responds to both a fatty acid and a pore-forming channel protein to induce the release of encapsulated cargo. We explored how membrane composition affects the functional assembly of α-hemolysin into phospholipid vesicles as a function of oleic acid content and α-hemolysin concentration. We then showed that we could induce α-hemolysin assembly when we added oleic acid micelles to a specific composition of phospholipid vesicles. Finally, we demonstrated that our membrane AND gate could be coupled to a gene expression system. Our study provides a new method to control the temporal dynamics of vesicle permeability by controlling when the functional assembly of a channel protein into synthetic vesicles occurs. Furthermore, a membrane AND gate that utilizes membrane-associating biomolecules introduces a new way to implement Boolean logic that should complement genetic logic circuits and ultimately enhance the capabilities of artificial cellular systems.

Original languageEnglish (US)
Pages (from-to)1224-1230
Number of pages7
JournalACS synthetic biology
Volume8
Issue number6
DOIs
StatePublished - Jun 21 2019

Fingerprint

Artificial Cells
Membranes
Hemolysin Proteins
Oleic acid
Phospholipids
Oleic Acid
Proteins
Logic circuits
Micelles
Biomolecules
Chemical analysis
Fatty acids
Gene expression
Permeability
Fatty Acids
Gene Expression

Keywords

  • artificial cell
  • cell-free expression
  • membrane logic gate
  • protein-membrane interactions

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Hilburger, Claire E. ; Jacobs, Miranda L. ; Lewis, Kamryn R. ; Peruzzi, Justin A. ; Kamat, Neha P. / Controlling Secretion in Artificial Cells with a Membrane and Gate. In: ACS synthetic biology. 2019 ; Vol. 8, No. 6. pp. 1224-1230.
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Controlling Secretion in Artificial Cells with a Membrane and Gate. / Hilburger, Claire E.; Jacobs, Miranda L.; Lewis, Kamryn R.; Peruzzi, Justin A.; Kamat, Neha P.

In: ACS synthetic biology, Vol. 8, No. 6, 21.06.2019, p. 1224-1230.

Research output: Contribution to journalArticle

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