Abstract
The assembly of channel proteins into vesicle membranes is a useful strategy to control activities of vesicle-based systems. Here, we developed a membrane AND gate that responds to both a fatty acid and a pore-forming channel protein to induce the release of encapsulated cargo. We explored how membrane composition affects the functional assembly of α-hemolysin into phospholipid vesicles as a function of oleic acid content and α-hemolysin concentration. We then showed that we could induce α-hemolysin assembly when we added oleic acid micelles to a specific composition of phospholipid vesicles. Finally, we demonstrated that our membrane AND gate could be coupled to a gene expression system. Our study provides a new method to control the temporal dynamics of vesicle permeability by controlling when the functional assembly of a channel protein into synthetic vesicles occurs. Furthermore, a membrane AND gate that utilizes membrane-associating biomolecules introduces a new way to implement Boolean logic that should complement genetic logic circuits and ultimately enhance the capabilities of artificial cellular systems.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1224-1230 |
| Number of pages | 7 |
| Journal | ACS synthetic biology |
| Volume | 8 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 21 2019 |
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Keywords
- artificial cell
- cell-free expression
- membrane logic gate
- protein-membrane interactions
ASJC Scopus subject areas
- Biomedical Engineering
- Biochemistry, Genetics and Molecular Biology (miscellaneous)
Cite this
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Controlling Secretion in Artificial Cells with a Membrane and Gate. / Hilburger, Claire E.; Jacobs, Miranda L.; Lewis, Kamryn R.; Peruzzi, Justin A.; Kamat, Neha P.
In: ACS synthetic biology, Vol. 8, No. 6, 21.06.2019, p. 1224-1230.Research output: Contribution to journal › Article
TY - JOUR
T1 - Controlling Secretion in Artificial Cells with a Membrane and Gate
AU - Hilburger, Claire E.
AU - Jacobs, Miranda L.
AU - Lewis, Kamryn R.
AU - Peruzzi, Justin A.
AU - Kamat, Neha P.
PY - 2019/6/21
Y1 - 2019/6/21
N2 - The assembly of channel proteins into vesicle membranes is a useful strategy to control activities of vesicle-based systems. Here, we developed a membrane AND gate that responds to both a fatty acid and a pore-forming channel protein to induce the release of encapsulated cargo. We explored how membrane composition affects the functional assembly of α-hemolysin into phospholipid vesicles as a function of oleic acid content and α-hemolysin concentration. We then showed that we could induce α-hemolysin assembly when we added oleic acid micelles to a specific composition of phospholipid vesicles. Finally, we demonstrated that our membrane AND gate could be coupled to a gene expression system. Our study provides a new method to control the temporal dynamics of vesicle permeability by controlling when the functional assembly of a channel protein into synthetic vesicles occurs. Furthermore, a membrane AND gate that utilizes membrane-associating biomolecules introduces a new way to implement Boolean logic that should complement genetic logic circuits and ultimately enhance the capabilities of artificial cellular systems.
AB - The assembly of channel proteins into vesicle membranes is a useful strategy to control activities of vesicle-based systems. Here, we developed a membrane AND gate that responds to both a fatty acid and a pore-forming channel protein to induce the release of encapsulated cargo. We explored how membrane composition affects the functional assembly of α-hemolysin into phospholipid vesicles as a function of oleic acid content and α-hemolysin concentration. We then showed that we could induce α-hemolysin assembly when we added oleic acid micelles to a specific composition of phospholipid vesicles. Finally, we demonstrated that our membrane AND gate could be coupled to a gene expression system. Our study provides a new method to control the temporal dynamics of vesicle permeability by controlling when the functional assembly of a channel protein into synthetic vesicles occurs. Furthermore, a membrane AND gate that utilizes membrane-associating biomolecules introduces a new way to implement Boolean logic that should complement genetic logic circuits and ultimately enhance the capabilities of artificial cellular systems.
KW - artificial cell
KW - cell-free expression
KW - membrane logic gate
KW - protein-membrane interactions
UR - http://www.scopus.com/inward/record.url?scp=85067687838&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85067687838&partnerID=8YFLogxK
U2 - 10.1021/acssynbio.8b00435
DO - 10.1021/acssynbio.8b00435
M3 - Article
C2 - 31051071
AN - SCOPUS:85067687838
VL - 8
SP - 1224
EP - 1230
JO - ACS Synthetic Biology
JF - ACS Synthetic Biology
SN - 2161-5063
IS - 6
ER -