Convection-enhanced delivery of targeted quantum dot-immunoliposome hybrid nanoparticles to intracranial brain tumor models

Kevin C. Weng, Rintaro Hashizume, Charles O. Noble, Laura P. Serwer, Daryl C. Drummond, Dmitri B. Kirpotin, Anne M. Kuwabara, Lucy X. Chao, Fanqing F. Chen, Charles D. James, John W. Park*

*Corresponding author for this work

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Aim: The aim of this work is to evaluate combining targeting strategy and convection-enhanced delivery in brain tumor models by imaging quantum dot-immunoliposome hybrid nanoparticles. Materials & methods: An EGF receptor-targeted, quantum dot-immunoliposome hybrid nanoparticle (QD-IL) was synthesized. In vitro uptake was measured by flow cytometry and intracellular localization was imaged by confocal microscopy. In the in vivo study, QD-ILs were delivered to intracranial xenografts via convection-enhanced delivery and fluorescence was monitored noninvasively in real-time. Results: QD-ILs exhibited specific and efficient uptake in vitro and exhibited approximately 1.3- to 5.0-fold higher total fluorescence compared with nontargeted counterpart in intracranial brain tumor xenografts in vivo. Conclusion: QD-ILs serve as an effective imaging agent in vitro and in vivo, and the data suggest that ligand-directed liposomal nanoparticles in conjunction with convection-enhanced delivery may offer therapeutic benefits for glioblastoma treatment as a result of specific and efficient uptake by malignant cells. Original submitted 15 June 2012; Revised submitted 12 November 2012; Published online 30 April 201.

Original languageEnglish (US)
Pages (from-to)1913-1925
Number of pages13
JournalNanomedicine
Volume8
Issue number12
DOIs
StatePublished - Jan 1 2013

Keywords

  • brain tumor model
  • convection-enhanced delivery
  • drug delivery system
  • immunoliposome
  • in vivo imaging
  • nanomedicine
  • quantum dot
  • targeted cancer therapy
  • targeted nanoparticle

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)

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  • Cite this

    Weng, K. C., Hashizume, R., Noble, C. O., Serwer, L. P., Drummond, D. C., Kirpotin, D. B., Kuwabara, A. M., Chao, L. X., Chen, F. F., James, C. D., & Park, J. W. (2013). Convection-enhanced delivery of targeted quantum dot-immunoliposome hybrid nanoparticles to intracranial brain tumor models. Nanomedicine, 8(12), 1913-1925. https://doi.org/10.2217/nnm.12.209