Cooperative signaling between Slit2 and ephrin-A1 regulates a balance between angiogenesis and angiostasis

Charlene M. Dunaway, Yoonha Hwang, Craig W. Lindsley, Rebecca S. Cook, Jane Y. Wu, Mark Boothby, Jin Chen, Dana M. Brantley-Sieders

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


Slit proteins induce cytoskeletal remodeling through interaction with roundabout (Robo) receptors, regulating migration of neurons and nonneuronal cells, including leukocytes, tumor cells, and endothelium. The role of Slit2 in vascular remodeling, however, remains controversial, with reports of both pro- and antiangiogenic activity. We report here that cooperation between Slit2 and ephrin-A1 regulates a balance between the pro- and antiangiogenic functions of Slit2. While Slit2 promotes angiogenesis in culture and in vivo as a single agent, Slit2 potently inhibits angiogenic remodeling in the presence of ephrin-A1. Slit2 stimulates angiogenesis through mTORC2-dependent activation of Akt and Rac GTPase, the activities of which are inhibited in the presence of ephrin-A1. Activated Rac or Akt partially rescues vascular assembly and motility in costimulated endothelium. Taken together, these data suggest that Slit2 differentially regulates angiogenesis in the context of ephrin-A1, providing a plausible mechanism for the pro- versus antiangiogenic functions of Slit2. Our results suggest that the complex roles of Slit-Robo signaling in angiogenesis involve context-dependent mechanisms.

Original languageEnglish (US)
Pages (from-to)404-416
Number of pages13
JournalMolecular and cellular biology
Issue number3
StatePublished - Feb 2011

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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