Coordinated Regulation of Polycomb Group Complexes through microRNAs in Cancer

Qi Cao; Ram Shankar Mani; Bushra Ateeq; Saravana M. Dhanasekaran; Irfan A. Asangani; John R. Prensner; Jung H. Kim; J. Chad Brenner; Xiaojun Jing; Xuhong Cao; Rui Wang; Yong Li; Arun Dahiya; Lei Wang; Mithil Pandhi; Robert J. Lonigro; Yi Mi Wu; Scott A. Tomlins; Nallasivam Palanisamy; Zhaohui Qin; Jindan Yu; Christopher A. Maher; Sooryanarayana Varambally; Arul M. Chinnaiyan

doi:10.1016/j.ccr.2011.06.016

Coordinated Regulation of Polycomb Group Complexes through microRNAs in Cancer

Qi Cao, Ram Shankar Mani, Bushra Ateeq, Saravana M. Dhanasekaran, Irfan A. Asangani, John R. Prensner, Jung H. Kim, J. Chad Brenner, Xiaojun Jing, Xuhong Cao, Rui Wang, Yong Li, Arun Dahiya, Lei Wang, Mithil Pandhi, Robert J. Lonigro, Yi Mi Wu, Scott A. Tomlins, Nallasivam Palanisamy, Zhaohui QinJindan Yu, Christopher A. Maher, Sooryanarayana Varambally, Arul M. Chinnaiyan^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

192 Scopus citations

Abstract

Polycomb Repressive Complexes (PRC1 and PRC2)-mediated epigenetic regulation is critical for maintaining cellular homeostasis. Members of Polycomb Group (PcG) proteins including EZH2, a PRC2 component, are upregulated in various cancer types, implicating their role in tumorigenesis. Here, we have identified several microRNAs (miRNAs) that are repressed by EZH2. These miRNAs, in turn, regulate the expression of PRC1 proteins BMI1 and RING2. We found that ectopic overexpression of EZH2-regulated miRNAs attenuated cancer cell growth and invasiveness, and abrogated cancer stem cell properties. Importantly, expression analysis revealed an inverse correlation between miRNA and PRC protein levels in cell culture and prostate cancer tissues. Taken together, our data have uncovered a coordinate regulation of PRC1 and PRC2 activities that is mediated by miRNAs.

Original language	English (US)
Pages (from-to)	187-199
Number of pages	13
Journal	Cancer cell
Volume	20
Issue number	2
DOIs	https://doi.org/10.1016/j.ccr.2011.06.016
State	Published - Aug 16 2011

Funding

We thank Xia Jiang, Javed Siddiqui, Wei Yan, Bo Han, Khalid Suleman, Rohit Mehra, Rupal Shastri, and Joy E. Tsai for technical assistance, Victor E. Marquez for providing DZNep, Michigan Center for hES Cell Research for H7 RNA and qPCR, the University of Michigan Vector Core for generating adenovirus and lentivirus, Kenneth J. Pienta, Xiaosong Wang, and Shanker Kalyana-Sundaram for discussions, and Jyoti Athanikar and Karen Giles for critically reading the manuscript and submission documents. This work is supported in part by the Early Detection Research Network UO1 CA111275, Prostate SPORE P50CA69568 and P50CA090386, and National Institutes of Health (R01CA132874, R01CA157845). A.M.C. is supported by the Doris Duke Charitable Foundation Clinical Scientist Award, Burroughs Welcome Foundation Award in Clinical Translational Research, the Prostate Cancer Foundation (PCF) and the Howard Hughes Medical Institute. A.M.C. is an American Cancer Society Research Professor. Q.C., J.R.P., and J.C.B. are supported by U.S. Department of Defense (PC094725 to Q.C.; PC094290 to J.R.P.; BC083217 to J.C.B.); R.-S.M., S.A.T., and C.A.M. are supported by Young Investigator Awards from the PCF; B.A. is supported by the Genentech Foundation Postdoctoral Fellowship and Young Investigator Award from the Expedition Inspiration Fund for Breast Cancer Research. N.P. is supported by the University of Michigan Prostate Cancer SPORE Career Development award. Z.Q. is supported by National Institutes of Health (7R01HG005119-02). J.Y. is supported by U.S. Department of Defense (PC080665) and National Institutes of Health (5R00CA129565); S.V. is supported by National Institutes of Health (R01CA157845) and Prostate Cancer SPORE Career Development award. Q.C. and A.M.C. designed the experiments. Q.C., R.-S.M., B.A., S.M.D., I.A.A., J.R.P., J.H.K., J.C.B., X.J., X.C., R.W., Y.L., A.D., L.W., M.P., Y.-M.W., S.A.T., N.P., J.Y., and S.V. performed the experimental work. R.J.L., Z.Q., and C.A.M. performed statistical analysis on miRNA and gene expression data. Q.C. and A.M.C. wrote the paper. All authors discussed the results and commented on the manuscript.

ASJC Scopus subject areas

Oncology
Cell Biology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ccr.2011.06.016

Cite this

Cao, Q., Mani, R. S., Ateeq, B., Dhanasekaran, S. M., Asangani, I. A., Prensner, J. R., Kim, J. H., Brenner, J. C., Jing, X., Cao, X., Wang, R., Li, Y., Dahiya, A., Wang, L., Pandhi, M., Lonigro, R. J., Wu, Y. M., Tomlins, S. A., Palanisamy, N., ... Chinnaiyan, A. M. (2011). Coordinated Regulation of Polycomb Group Complexes through microRNAs in Cancer. Cancer cell, 20(2), 187-199. https://doi.org/10.1016/j.ccr.2011.06.016

@article{ecaf012cf2a9407b8ca51531b2f2c0c0,

title = "Coordinated Regulation of Polycomb Group Complexes through microRNAs in Cancer",

abstract = "Polycomb Repressive Complexes (PRC1 and PRC2)-mediated epigenetic regulation is critical for maintaining cellular homeostasis. Members of Polycomb Group (PcG) proteins including EZH2, a PRC2 component, are upregulated in various cancer types, implicating their role in tumorigenesis. Here, we have identified several microRNAs (miRNAs) that are repressed by EZH2. These miRNAs, in turn, regulate the expression of PRC1 proteins BMI1 and RING2. We found that ectopic overexpression of EZH2-regulated miRNAs attenuated cancer cell growth and invasiveness, and abrogated cancer stem cell properties. Importantly, expression analysis revealed an inverse correlation between miRNA and PRC protein levels in cell culture and prostate cancer tissues. Taken together, our data have uncovered a coordinate regulation of PRC1 and PRC2 activities that is mediated by miRNAs.",

author = "Qi Cao and Mani, \{Ram Shankar\} and Bushra Ateeq and Dhanasekaran, \{Saravana M.\} and Asangani, \{Irfan A.\} and Prensner, \{John R.\} and Kim, \{Jung H.\} and Brenner, \{J. Chad\} and Xiaojun Jing and Xuhong Cao and Rui Wang and Yong Li and Arun Dahiya and Lei Wang and Mithil Pandhi and Lonigro, \{Robert J.\} and Wu, \{Yi Mi\} and Tomlins, \{Scott A.\} and Nallasivam Palanisamy and Zhaohui Qin and Jindan Yu and Maher, \{Christopher A.\} and Sooryanarayana Varambally and Chinnaiyan, \{Arul M.\}",

note = "Funding Information: We thank Xia Jiang, Javed Siddiqui, Wei Yan, Bo Han, Khalid Suleman, Rohit Mehra, Rupal Shastri, and Joy E. Tsai for technical assistance, Victor E. Marquez for providing DZNep, Michigan Center for hES Cell Research for H7 RNA and qPCR, the University of Michigan Vector Core for generating adenovirus and lentivirus, Kenneth J. Pienta, Xiaosong Wang, and Shanker Kalyana-Sundaram for discussions, and Jyoti Athanikar and Karen Giles for critically reading the manuscript and submission documents. This work is supported in part by the Early Detection Research Network UO1 CA111275, Prostate SPORE P50CA69568 and P50CA090386, and National Institutes of Health (R01CA132874, R01CA157845). A.M.C. is supported by the Doris Duke Charitable Foundation Clinical Scientist Award, Burroughs Welcome Foundation Award in Clinical Translational Research, the Prostate Cancer Foundation (PCF) and the Howard Hughes Medical Institute. A.M.C. is an American Cancer Society Research Professor. Q.C., J.R.P., and J.C.B. are supported by U.S. Department of Defense (PC094725 to Q.C.; PC094290 to J.R.P.; BC083217 to J.C.B.); R.-S.M., S.A.T., and C.A.M. are supported by Young Investigator Awards from the PCF; B.A. is supported by the Genentech Foundation Postdoctoral Fellowship and Young Investigator Award from the Expedition Inspiration Fund for Breast Cancer Research. N.P. is supported by the University of Michigan Prostate Cancer SPORE Career Development award. Z.Q. is supported by National Institutes of Health (7R01HG005119-02). J.Y. is supported by U.S. Department of Defense (PC080665) and National Institutes of Health (5R00CA129565); S.V. is supported by National Institutes of Health (R01CA157845) and Prostate Cancer SPORE Career Development award. Q.C. and A.M.C. designed the experiments. Q.C., R.-S.M., B.A., S.M.D., I.A.A., J.R.P., J.H.K., J.C.B., X.J., X.C., R.W., Y.L., A.D., L.W., M.P., Y.-M.W., S.A.T., N.P., J.Y., and S.V. performed the experimental work. R.J.L., Z.Q., and C.A.M. performed statistical analysis on miRNA and gene expression data. Q.C. and A.M.C. wrote the paper. All authors discussed the results and commented on the manuscript. ",

year = "2011",

month = aug,

day = "16",

doi = "10.1016/j.ccr.2011.06.016",

language = "English (US)",

volume = "20",

pages = "187--199",

journal = "Cancer cell",

issn = "1535-6108",

publisher = "Cell Press",

number = "2",

}

Cao, Q, Mani, RS, Ateeq, B, Dhanasekaran, SM, Asangani, IA, Prensner, JR, Kim, JH, Brenner, JC, Jing, X, Cao, X, Wang, R, Li, Y, Dahiya, A, Wang, L, Pandhi, M, Lonigro, RJ, Wu, YM, Tomlins, SA, Palanisamy, N, Qin, Z, Yu, J, Maher, CA, Varambally, S & Chinnaiyan, AM 2011, 'Coordinated Regulation of Polycomb Group Complexes through microRNAs in Cancer', Cancer cell, vol. 20, no. 2, pp. 187-199. https://doi.org/10.1016/j.ccr.2011.06.016

TY - JOUR

T1 - Coordinated Regulation of Polycomb Group Complexes through microRNAs in Cancer

AU - Cao, Qi

AU - Mani, Ram Shankar

AU - Ateeq, Bushra

AU - Dhanasekaran, Saravana M.

AU - Asangani, Irfan A.

AU - Prensner, John R.

AU - Kim, Jung H.

AU - Brenner, J. Chad

AU - Jing, Xiaojun

AU - Cao, Xuhong

AU - Wang, Rui

AU - Li, Yong

AU - Dahiya, Arun

AU - Wang, Lei

AU - Pandhi, Mithil

AU - Lonigro, Robert J.

AU - Wu, Yi Mi

AU - Tomlins, Scott A.

AU - Palanisamy, Nallasivam

AU - Qin, Zhaohui

AU - Yu, Jindan

AU - Maher, Christopher A.

AU - Varambally, Sooryanarayana

AU - Chinnaiyan, Arul M.

N1 - Funding Information: We thank Xia Jiang, Javed Siddiqui, Wei Yan, Bo Han, Khalid Suleman, Rohit Mehra, Rupal Shastri, and Joy E. Tsai for technical assistance, Victor E. Marquez for providing DZNep, Michigan Center for hES Cell Research for H7 RNA and qPCR, the University of Michigan Vector Core for generating adenovirus and lentivirus, Kenneth J. Pienta, Xiaosong Wang, and Shanker Kalyana-Sundaram for discussions, and Jyoti Athanikar and Karen Giles for critically reading the manuscript and submission documents. This work is supported in part by the Early Detection Research Network UO1 CA111275, Prostate SPORE P50CA69568 and P50CA090386, and National Institutes of Health (R01CA132874, R01CA157845). A.M.C. is supported by the Doris Duke Charitable Foundation Clinical Scientist Award, Burroughs Welcome Foundation Award in Clinical Translational Research, the Prostate Cancer Foundation (PCF) and the Howard Hughes Medical Institute. A.M.C. is an American Cancer Society Research Professor. Q.C., J.R.P., and J.C.B. are supported by U.S. Department of Defense (PC094725 to Q.C.; PC094290 to J.R.P.; BC083217 to J.C.B.); R.-S.M., S.A.T., and C.A.M. are supported by Young Investigator Awards from the PCF; B.A. is supported by the Genentech Foundation Postdoctoral Fellowship and Young Investigator Award from the Expedition Inspiration Fund for Breast Cancer Research. N.P. is supported by the University of Michigan Prostate Cancer SPORE Career Development award. Z.Q. is supported by National Institutes of Health (7R01HG005119-02). J.Y. is supported by U.S. Department of Defense (PC080665) and National Institutes of Health (5R00CA129565); S.V. is supported by National Institutes of Health (R01CA157845) and Prostate Cancer SPORE Career Development award. Q.C. and A.M.C. designed the experiments. Q.C., R.-S.M., B.A., S.M.D., I.A.A., J.R.P., J.H.K., J.C.B., X.J., X.C., R.W., Y.L., A.D., L.W., M.P., Y.-M.W., S.A.T., N.P., J.Y., and S.V. performed the experimental work. R.J.L., Z.Q., and C.A.M. performed statistical analysis on miRNA and gene expression data. Q.C. and A.M.C. wrote the paper. All authors discussed the results and commented on the manuscript.

PY - 2011/8/16

Y1 - 2011/8/16

N2 - Polycomb Repressive Complexes (PRC1 and PRC2)-mediated epigenetic regulation is critical for maintaining cellular homeostasis. Members of Polycomb Group (PcG) proteins including EZH2, a PRC2 component, are upregulated in various cancer types, implicating their role in tumorigenesis. Here, we have identified several microRNAs (miRNAs) that are repressed by EZH2. These miRNAs, in turn, regulate the expression of PRC1 proteins BMI1 and RING2. We found that ectopic overexpression of EZH2-regulated miRNAs attenuated cancer cell growth and invasiveness, and abrogated cancer stem cell properties. Importantly, expression analysis revealed an inverse correlation between miRNA and PRC protein levels in cell culture and prostate cancer tissues. Taken together, our data have uncovered a coordinate regulation of PRC1 and PRC2 activities that is mediated by miRNAs.

AB - Polycomb Repressive Complexes (PRC1 and PRC2)-mediated epigenetic regulation is critical for maintaining cellular homeostasis. Members of Polycomb Group (PcG) proteins including EZH2, a PRC2 component, are upregulated in various cancer types, implicating their role in tumorigenesis. Here, we have identified several microRNAs (miRNAs) that are repressed by EZH2. These miRNAs, in turn, regulate the expression of PRC1 proteins BMI1 and RING2. We found that ectopic overexpression of EZH2-regulated miRNAs attenuated cancer cell growth and invasiveness, and abrogated cancer stem cell properties. Importantly, expression analysis revealed an inverse correlation between miRNA and PRC protein levels in cell culture and prostate cancer tissues. Taken together, our data have uncovered a coordinate regulation of PRC1 and PRC2 activities that is mediated by miRNAs.

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U2 - 10.1016/j.ccr.2011.06.016

DO - 10.1016/j.ccr.2011.06.016

M3 - Article

C2 - 21840484

AN - SCOPUS:80051590718

SN - 1535-6108

VL - 20

SP - 187

EP - 199

JO - Cancer cell

JF - Cancer cell

IS - 2

ER -

Coordinated Regulation of Polycomb Group Complexes through microRNAs in Cancer

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