Correlation of manual semi-quantitative and automated quantitative Ki-67 proliferative index with OncotypeDX™ recurrence score in invasive breast carcinoma

Brian S. Finkelman, Amanda Meindl, Carissa Laboy, Brannan B. Griffin, Suguna P. Narayan, Rachel Brancamp, Kalliopi P. Siziopikou, Jennifer L. Pincus, Luis Z. Blanco*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND: Ki-67 immunohistochemistry (IHC) staining is a widely used cancer proliferation assay; however, its limitations could be improved with automated scoring. The OncotypeDX™ Recurrence Score (ORS), which primarily evaluates cancer proliferation genes, is a prognostic indicator for breast cancer chemotherapy response; however, it is more expensive and slower than Ki-67. OBJECTIVE: To compare manual Ki-67 (mKi-67) with automated Ki-67 (aKi-67) algorithm results based on manually selected Ki-67 'hot spots' in breast cancer, and correlate both with ORS. METHODS: 105 invasive breast carcinoma cases from 100 patients at our institution (2011-2013) with available ORS were evaluated. Concordance was assessed via Cohen's Kappa (κ). RESULTS: 57/105 cases showed agreement between mKi-67 and aKi-67 (κ 0.31, 95% CI 0.18-0.45), with 41 cases overestimated by aKi-67. Concordance was higher when estimated on the same image (κ 0.53, 95% CI 0.37-0.69). Concordance between mKi-67 score and ORS was fair (κ 0.27, 95% CI 0.11-0.42), and concordance between aKi-67 and ORS was poor (κ 0.10, 95% CI -0.03-0.23). CONCLUSIONS: These results highlight the limits of Ki-67 algorithms that use manual 'hot spot' selection. Due to suboptimal concordance, Ki-67 is likely most useful as a complement to, rather than a surrogate for ORS, regardless of scoring method.

Original languageEnglish (US)
Pages (from-to)55-65
Number of pages11
JournalBreast Disease
Volume41
Issue number1
DOIs
StatePublished - 2022

Keywords

  • Breast carcinoma
  • Ki-67
  • OncotypeDX
  • digital image analysis

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint

Dive into the research topics of 'Correlation of manual semi-quantitative and automated quantitative Ki-67 proliferative index with OncotypeDX™ recurrence score in invasive breast carcinoma'. Together they form a unique fingerprint.

Cite this