Correlation of RNA binding affinity of avian oncornavirus p19 proteins with the extent of processing of virus genome RNA in cells

J. P. Leis, P. Scheible, R. E. Smith

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

We purified the p19 proteins from the Prague C strain of Rous sarcoma virus, avian myeloblastosis virus, B77 sarcoma virus, myeloblastosis-associated virus-2(0), and PR-E 95-C virus and measured their binding affinities for 60S viral RNA by the nitrocellulose filter binding technique. The apparent association constants of the p19 proteins from Rous sarcoma virus Prague C, avian myeloblastosis virus, and B77 sarcoma virus for homologous and heterologous 60S RNAs were similar (1.5 x 1011 to 2.6 x 1011 liters/mol), whereas those of myeloblastosis-associated virus-2(0) and PR-E 95-C virus were 10-fold lower. The sizes and relative amounts of the virus-specific polyadenylic acid-containing RNAs in the cytoplasms of cells infected with Rous sarcoma virus Prague C, myeloblastosis-associated virus-2(0), and PR-E 95-C virus were determined by fractionating the RNAs on agarose gels containing methylmercury hydroxide, transferring them to diazobenzyloxymethyl paper and hybridizing them to a 70-nucleotide complementary DNA probe. In cells infected with Rous sarcoma virus Prague C we detected 3.4 x 106-, 1.9 x 106-, and 1.1 x 106-dalton RNAs, in PR-E 95-C virus infected cells we detected 3.4 x 106-, 1.9 x 106- and 0.7 x 106-dalton RNAs, and in cells infected with myeloblastosis-associated virus-2(0) we detected 3 x 106- and 1.3 x 106-dalton RNAs. Each of these RNA species contained RNA sequences derived from the 5' terminus of genome-length RNA, as evidenced by hybridization with the 5' 70-nucleotide complementary DNA. The ratios of subgenomic mRNA's to genome-length RNAs in cells infected with myeloblastosis-associated virus-2(0) and PR-E 95-C virus were three- to five-fold higher than the ratio in cells infected with Rous sarcoma virus Prague C. These results suggest that more processing of viral RNA in infected cells if correlated with lower binding affinities of the p19 protein for viral RNA, and the are consistent with the hypothesis that the p19 protein controls processing of viral RNA in cells.

Original languageEnglish (US)
Pages (from-to)722-731
Number of pages10
JournalJournal of virology
Volume35
Issue number3
DOIs
StatePublished - 1980

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Fingerprint Dive into the research topics of 'Correlation of RNA binding affinity of avian oncornavirus p19 proteins with the extent of processing of virus genome RNA in cells'. Together they form a unique fingerprint.

Cite this