Counterion distribution surrounding spherical nucleic acid-au nanoparticle conjugates probed by small-angle x-ray scattering

Sumit Kewalramani, Jos W. Zwanikken, Robert J. Macfarlane, Cheuk Yui Leung, Monica Olvera De La Cruz, Chad A. Mirkin, Michael J. Bedzyk*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The radial distribution of monovalent cations surrounding spherical nucleic acid-Au nanoparticle conjugates (SNA-AuNPs) is determined by in situ small-angle x-ray scattering (SAXS) and classical density functional theory (DFT) calculations. Small differences in SAXS intensity profiles from SNA-AuNPs dispersed in a series of solutions containing different monovalent ions (Na +, K+, Rb+, or Cs+) are measured. Using the "heavy ion replacement" SAXS (HIRSAXS) approach, we extract the cation-distribution-dependent contribution to the SAXS intensity and show that it agrees with DFT predictions. The experiment-theory comparisons reveal the radial distribution of cations as well as the conformation of the DNA in the SNA shell. The analysis shows an enhancement to the average cation concentration in the SNA shell that can be up to 15-fold, depending on the bulk solution ionic concentration. The study demonstrates the feasibility of HIRSAXS in probing the distribution of monovalent cations surrounding nanoparticles with an electron dense core (e.g., metals).

Original languageEnglish (US)
Pages (from-to)11301-11309
Number of pages9
JournalACS nano
Volume7
Issue number12
DOIs
StatePublished - Dec 23 2013

Keywords

  • DNA-coated gold nanoparticles
  • counterion distribution
  • density functional theory
  • small-angle X-ray scattering

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Fingerprint Dive into the research topics of 'Counterion distribution surrounding spherical nucleic acid-au nanoparticle conjugates probed by small-angle x-ray scattering'. Together they form a unique fingerprint.

Cite this