@article{cabec745f6a74d8da7861a7395ce1bf7,
title = "Covalent incorporation of selenium into oligonucleotides for X-ray crystal structure determination via MAD: Proof of principle",
abstract = "Selenium was incorporated into an oligodeoxynucleotide in the form of 2′-methylseleno-uridine (USe). The X-ray crystal structure of the duplex [d(GCGTA)USed(ACGC)]2 was determined by the multiwavelength anomalous dispersion (MAD) technique and refined to a resolution of 1.3 {\AA}, demonstrating that selenium can selectively substitute oxygen in DNA and that the resulting compounds are chemically stable. Since derivatization at the 2′-α-position with selenium does not affect the preference of the sugar for the C3′-endo conformation, this strategy is suitable for incorporating selenium into RNA. The availability of selenium-containing nucleic acids for crystallographic phasing offers an attractive alternative to the commonly used halogenated pyrimidins.",
keywords = "Anomalous dispersion, DNA, MAD phasing, Nucleic acids, RNA, Selenium labeling, X-ray crystallography",
author = "Marianna Teplova and Wilds, {Christopher J.} and Zdzislaw Wawrzak and Valentina Tereshko and Quan Du and Nicolas Carrasco and Zhen Huang and Martin Egli",
note = "Funding Information: Financial support from the National Institutes of Health (grant GM-55237 to ME) is gratefully acknowledged. ZH is supported by PSC-CUNY research awards (69674-00-29 and 62392-00-31) and the New Research Dimension Fund and CJW is the recipient of a post-graduate scholarship from the National Science and Engineering Research Council of Canada (NSERC). We are grateful to Professor Paul Miller, Johns Hopkins University, and Dr. Anne Noronha for their assistance with thermal melting experiments. Portions of this work were performed at the DuPont–Northwestern–Dow collaborative access team (DND-CAT) Synchrotron Research Center located at Sector 5 of the Advanced Photon Source. DND-CAT is supported by the E.I. DuPont de Nemours & Co., The Dow Chemical Company, the US National Science Foundation through Grant DMR-9304725 and the State of Illinois through the Department of Commerce and the Board of Higher Education Grant IBHE HECA NWU 96. Use of the Advanced Photon Source was supported by the US Department of Energy, Basic Energy Sciences, Office of Energy Research under Contract No. W-31-102-Eng-38.",
year = "2002",
month = sep,
day = "1",
doi = "10.1016/S0300-9084(02)01440-2",
language = "English (US)",
volume = "84",
pages = "849--858",
journal = "Biochimie",
issn = "0300-9084",
publisher = "Elsevier B.V.",
number = "9",
}