Abstract
CpG oligodeoxynucleotides (CpG ODN) are known to elicit Th1 immune responses via TLR9. However, the precise mechanisms through which B cells are involved in this phenomenon are not fully understood. We investigated the effect of CpG ODN on the induction of Th1-chemoattractant CXCR3 chemokines, IP-10, Mig, and I-TAC, in B cells. Cells from the RPMI 8226 human B cell line and human peripheral B cells were stimulated with three distinct classes of CpG ODN. As a result, CXCR3 chemokines were strongly up-regulated by CpG-B and CpG-C, but only weakly by CpG-A. Though CXCR3 chemokines are known to be induced by IFNs, blocking mAbs against IFN receptors did not inhibit their induction by CpG-B. Induction of CXCR3 chemokines was blocked by two NF-κB inhibitors and a p38 inhibitor. These results strongly suggest that CXCR3 chemokines are directly induced by CpG ODN via NF-κB- and p38-dependent pathways in human B cells.
Original language | English (US) |
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Pages (from-to) | 1139-1147 |
Number of pages | 9 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 320 |
Issue number | 4 |
DOIs | |
State | Published - Aug 6 2004 |
Funding
The authors also thank Ms. Noriko Hashimoto (National Research Institute for Child Health and Development) for skillful technical assistance. This work was supported in part by a grant from the Organization for Pharmaceutical Safety and Research and the Ministry of Health, Labour and Welfare (the Millennium Genome Project, MPJ-5), and by a grant from RIKEN Research Center for Allergy and Immunology.
Keywords
- B cells
- CXCR3 chemokines
- CpG ODN
- I-TAC
- IP-10
- Mig
- TLR9
ASJC Scopus subject areas
- Molecular Biology
- Biophysics
- Biochemistry
- Cell Biology