CpG oligodeoxynucleotides directly induce CXCR3 chemokines in human B cells

Atsushi Kato; Takahisa Ogasawara; Toshiki Homma; Jonathan Batchelor; Shosuke Imai; Hiroshi Wakiguchi; Hirohisa Saito; Kenji Matsumoto

doi:10.1016/j.bbrc.2004.06.059

CpG oligodeoxynucleotides directly induce CXCR3 chemokines in human B cells

Atsushi Kato, Takahisa Ogasawara, Toshiki Homma, Jonathan Batchelor, Shosuke Imai, Hiroshi Wakiguchi, Hirohisa Saito, Kenji Matsumoto^*

^*Corresponding author for this work

Medicine, Allergy Division

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

CpG oligodeoxynucleotides (CpG ODN) are known to elicit Th1 immune responses via TLR9. However, the precise mechanisms through which B cells are involved in this phenomenon are not fully understood. We investigated the effect of CpG ODN on the induction of Th1-chemoattractant CXCR3 chemokines, IP-10, Mig, and I-TAC, in B cells. Cells from the RPMI 8226 human B cell line and human peripheral B cells were stimulated with three distinct classes of CpG ODN. As a result, CXCR3 chemokines were strongly up-regulated by CpG-B and CpG-C, but only weakly by CpG-A. Though CXCR3 chemokines are known to be induced by IFNs, blocking mAbs against IFN receptors did not inhibit their induction by CpG-B. Induction of CXCR3 chemokines was blocked by two NF-κB inhibitors and a p38 inhibitor. These results strongly suggest that CXCR3 chemokines are directly induced by CpG ODN via NF-κB- and p38-dependent pathways in human B cells.

Original language	English (US)
Pages (from-to)	1139-1147
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	320
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2004.06.059
State	Published - Aug 6 2004

Funding

The authors also thank Ms. Noriko Hashimoto (National Research Institute for Child Health and Development) for skillful technical assistance. This work was supported in part by a grant from the Organization for Pharmaceutical Safety and Research and the Ministry of Health, Labour and Welfare (the Millennium Genome Project, MPJ-5), and by a grant from RIKEN Research Center for Allergy and Immunology.

Keywords

B cells
CXCR3 chemokines
CpG ODN
I-TAC
IP-10
Mig
TLR9

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry
Cell Biology

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title = "CpG oligodeoxynucleotides directly induce CXCR3 chemokines in human B cells",

abstract = "CpG oligodeoxynucleotides (CpG ODN) are known to elicit Th1 immune responses via TLR9. However, the precise mechanisms through which B cells are involved in this phenomenon are not fully understood. We investigated the effect of CpG ODN on the induction of Th1-chemoattractant CXCR3 chemokines, IP-10, Mig, and I-TAC, in B cells. Cells from the RPMI 8226 human B cell line and human peripheral B cells were stimulated with three distinct classes of CpG ODN. As a result, CXCR3 chemokines were strongly up-regulated by CpG-B and CpG-C, but only weakly by CpG-A. Though CXCR3 chemokines are known to be induced by IFNs, blocking mAbs against IFN receptors did not inhibit their induction by CpG-B. Induction of CXCR3 chemokines was blocked by two NF-κB inhibitors and a p38 inhibitor. These results strongly suggest that CXCR3 chemokines are directly induced by CpG ODN via NF-κB- and p38-dependent pathways in human B cells.",

keywords = "B cells, CXCR3 chemokines, CpG ODN, I-TAC, IP-10, Mig, TLR9",

author = "Atsushi Kato and Takahisa Ogasawara and Toshiki Homma and Jonathan Batchelor and Shosuke Imai and Hiroshi Wakiguchi and Hirohisa Saito and Kenji Matsumoto",

note = "Funding Information: The authors also thank Ms. Noriko Hashimoto (National Research Institute for Child Health and Development) for skillful technical assistance. This work was supported in part by a grant from the Organization for Pharmaceutical Safety and Research and the Ministry of Health, Labour and Welfare (the Millennium Genome Project, MPJ-5), and by a grant from RIKEN Research Center for Allergy and Immunology.",

year = "2004",

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doi = "10.1016/j.bbrc.2004.06.059",

language = "English (US)",

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pages = "1139--1147",

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T1 - CpG oligodeoxynucleotides directly induce CXCR3 chemokines in human B cells

AU - Kato, Atsushi

AU - Ogasawara, Takahisa

AU - Homma, Toshiki

AU - Batchelor, Jonathan

AU - Imai, Shosuke

AU - Wakiguchi, Hiroshi

AU - Saito, Hirohisa

AU - Matsumoto, Kenji

N1 - Funding Information: The authors also thank Ms. Noriko Hashimoto (National Research Institute for Child Health and Development) for skillful technical assistance. This work was supported in part by a grant from the Organization for Pharmaceutical Safety and Research and the Ministry of Health, Labour and Welfare (the Millennium Genome Project, MPJ-5), and by a grant from RIKEN Research Center for Allergy and Immunology.

PY - 2004/8/6

Y1 - 2004/8/6

N2 - CpG oligodeoxynucleotides (CpG ODN) are known to elicit Th1 immune responses via TLR9. However, the precise mechanisms through which B cells are involved in this phenomenon are not fully understood. We investigated the effect of CpG ODN on the induction of Th1-chemoattractant CXCR3 chemokines, IP-10, Mig, and I-TAC, in B cells. Cells from the RPMI 8226 human B cell line and human peripheral B cells were stimulated with three distinct classes of CpG ODN. As a result, CXCR3 chemokines were strongly up-regulated by CpG-B and CpG-C, but only weakly by CpG-A. Though CXCR3 chemokines are known to be induced by IFNs, blocking mAbs against IFN receptors did not inhibit their induction by CpG-B. Induction of CXCR3 chemokines was blocked by two NF-κB inhibitors and a p38 inhibitor. These results strongly suggest that CXCR3 chemokines are directly induced by CpG ODN via NF-κB- and p38-dependent pathways in human B cells.

AB - CpG oligodeoxynucleotides (CpG ODN) are known to elicit Th1 immune responses via TLR9. However, the precise mechanisms through which B cells are involved in this phenomenon are not fully understood. We investigated the effect of CpG ODN on the induction of Th1-chemoattractant CXCR3 chemokines, IP-10, Mig, and I-TAC, in B cells. Cells from the RPMI 8226 human B cell line and human peripheral B cells were stimulated with three distinct classes of CpG ODN. As a result, CXCR3 chemokines were strongly up-regulated by CpG-B and CpG-C, but only weakly by CpG-A. Though CXCR3 chemokines are known to be induced by IFNs, blocking mAbs against IFN receptors did not inhibit their induction by CpG-B. Induction of CXCR3 chemokines was blocked by two NF-κB inhibitors and a p38 inhibitor. These results strongly suggest that CXCR3 chemokines are directly induced by CpG ODN via NF-κB- and p38-dependent pathways in human B cells.

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KW - CpG ODN

KW - I-TAC

KW - IP-10

KW - Mig

KW - TLR9

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CpG oligodeoxynucleotides directly induce CXCR3 chemokines in human B cells

Abstract

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