CpG oligodeoxynucleotides directly induce CXCR3 chemokines in human B cells

Atsushi Kato, Takahisa Ogasawara, Toshiki Homma, Jonathan Batchelor, Shosuke Imai, Hiroshi Wakiguchi, Hirohisa Saito, Kenji Matsumoto*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

CpG oligodeoxynucleotides (CpG ODN) are known to elicit Th1 immune responses via TLR9. However, the precise mechanisms through which B cells are involved in this phenomenon are not fully understood. We investigated the effect of CpG ODN on the induction of Th1-chemoattractant CXCR3 chemokines, IP-10, Mig, and I-TAC, in B cells. Cells from the RPMI 8226 human B cell line and human peripheral B cells were stimulated with three distinct classes of CpG ODN. As a result, CXCR3 chemokines were strongly up-regulated by CpG-B and CpG-C, but only weakly by CpG-A. Though CXCR3 chemokines are known to be induced by IFNs, blocking mAbs against IFN receptors did not inhibit their induction by CpG-B. Induction of CXCR3 chemokines was blocked by two NF-κB inhibitors and a p38 inhibitor. These results strongly suggest that CXCR3 chemokines are directly induced by CpG ODN via NF-κB- and p38-dependent pathways in human B cells.

Original languageEnglish (US)
Pages (from-to)1139-1147
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume320
Issue number4
DOIs
StatePublished - Aug 6 2004

Keywords

  • B cells
  • CXCR3 chemokines
  • CpG ODN
  • I-TAC
  • IP-10
  • Mig
  • TLR9

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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