Abstract
Cellular retinaldehyde binding protein (CRALBP) functions in the visual cycle and mutations in the RLBP1 gene can lead to blindness. RLBP1 promoter analyses have been pursued in vitro as an approach to deciphering the mechanism controlling cell-specific expression of CRALBP. Reporter activity of wildtype and mutant RLBP1 promoter constructs suggest that CRALBP transcriptional regulation may be similar in the ciliary epithelium (CE) and retinal pigment epithelium (RPE) but different in Müller cells. Results in RPE cells refine the location of an RLBP1 enhancer element to within -1826 to -1749 bp and a repressor element to within -702 to -635 bp.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 257-260 |
| Number of pages | 4 |
| Journal | Experimental eye research |
| Volume | 76 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 2003 |
Funding
Supported by NIH grants EY06603, EY14239, EY03523 and EY04873, a Research Center grant from The Foundation Fighting Blindness, a grant from Research to Prevent Blindness and funds from the Cleveland Clinic Foundation. The authors thank Drs. M. Tenniswood, F. Martin, D. Zack, J. Hurley and J. O'Sullivan, for helpful discussions, and S. Agarwal for technical assistance.
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience
- Ophthalmology