Cre recombinase activity specific to post natal, premeiotic male germ cells in transgenic mice

Patricia I. Sadate-Ngatchou, Christopher J. Payne, Andrea T. Dearth, Robert E. Braun*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

169 Scopus citations

Abstract

We have generated a transgenic mouse line, Tg(Stra8-cre)1Reb (Straβ-cre), which expresses improved Cre recombinase under the control of a 1.4 Kb promoter region of the germ cell-specific stimulated by retinoic acid gene 8 (Stra8). cre is expressed only in males beginning at postnatal day (P)3 in early-stage spermatogonia and is detected through preleptotene-stage spermatocytes. To further define when cre becomes active, we crossed Stra8-cre males with Tg(ACTB-Bgeo/GFP)21Lbe (Z/EG) reporter females and compared the expression of enhanced green fluorescent protein (EGFP) with the protein encoded by the zinc finger and BTB domain containing 16 (Zbtb16) gene, PLZF - a marker for undifferentiated spermatogonia. Co-expression of EGFP is observed in the majority of PLZF+ cells. We also tested recombination efficiency by mating Stra8-cre;Z/EG males and females with wild-type mice and examining EGFP expression in the offspring. Recombination is detected in >95% of Z/EG+ pups born to Stra8-cre;Z/EG fathers but in none of the offspring born to transgenic mothers, a verification that cre is not functional in females. The postnatal, premeiotic, male germ cell-specific activity of Stra8-cre makes this mouse line a unique resource to study testicular germ cell development.

Original languageEnglish (US)
Pages (from-to)738-742
Number of pages5
JournalGenesis
Volume46
Issue number12
DOIs
StatePublished - 2008

Keywords

  • Cre recombinase
  • Spermatocytes
  • Spermatogonia
  • Stra8 promoter
  • Z/EG

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

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