Cripto-1: An extracellular protein - Connecting the sequestered biological dots

Malgorzata Klauzinska; Daniel Bertolette; Sudhamsh Tippireddy; Luigi Strizzi; Peter C. Gray; Monica Gonzales; Meg Duroux; Menotti Ruvo; Christian Wechselberger; Nadia P. Castro; Maria Cristina Rangel; Annalia Focà; Annamaria Sandomenico; Mary J.C. Hendrix; David Salomon; Frank Cuttitta

doi:10.3109/03008207.2015.1077239

Cripto-1: An extracellular protein - Connecting the sequestered biological dots

Malgorzata Klauzinska^*, Daniel Bertolette, Sudhamsh Tippireddy, Luigi Strizzi, Peter C. Gray, Monica Gonzales, Meg Duroux, Menotti Ruvo, Christian Wechselberger, Nadia P. Castro, Maria Cristina Rangel, Annalia Focà, Annamaria Sandomenico, Mary J.C. Hendrix, David Salomon, Frank Cuttitta

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Cripto-1 (CR-1) is a multifunctional embryonic protein that is re-expressed during inflammation, wound repair, and malignant transformation. CR-1 can function either as a tethered co-receptor or shed as a free ligand underpinning its flexible role in cell physiology. CR-1 has been shown to mediate cell growth, migration, invasion, and induce epithelial to mesenchymal transition (EMT). The main signaling pathways mediating CR-1 effects include Nodal-dependent (Smad2/3) and Nodal-independent (Src/p44/42/Akt) signaling transduction pathways. In addition, there are several naturally occurring binding partner proteins (BPPs) for CR-1 that can either agonize or antagonize its bioactivity. We will review the collective role of CR-1 as an extracellular protein, discuss caveats to consider in developing a quantitation assay, define possible mechanistic avenues applicable for drug discovery, and report on our experimental approaches to overcome these problematic issues.

Original language	English (US)
Pages (from-to)	364-380
Number of pages	17
Journal	Connective tissue research
Volume	56
Issue number	5
DOIs	https://doi.org/10.3109/03008207.2015.1077239
State	Published - Sep 3 2015

Keywords

Autoantibodies
Cripto-1
Cripto-1 detection
drug discovery
inflammation

ASJC Scopus subject areas

Molecular Biology
Biochemistry
Rheumatology
Cell Biology
Orthopedics and Sports Medicine

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10.3109/03008207.2015.1077239

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Klauzinska, M., Bertolette, D., Tippireddy, S., Strizzi, L., Gray, P. C., Gonzales, M., Duroux, M., Ruvo, M., Wechselberger, C., Castro, N. P., Rangel, M. C., Focà, A., Sandomenico, A., Hendrix, M. J. C., Salomon, D., & Cuttitta, F. (2015). Cripto-1: An extracellular protein - Connecting the sequestered biological dots. Connective tissue research, 56(5), 364-380. https://doi.org/10.3109/03008207.2015.1077239

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title = "Cripto-1: An extracellular protein - Connecting the sequestered biological dots",

abstract = "Cripto-1 (CR-1) is a multifunctional embryonic protein that is re-expressed during inflammation, wound repair, and malignant transformation. CR-1 can function either as a tethered co-receptor or shed as a free ligand underpinning its flexible role in cell physiology. CR-1 has been shown to mediate cell growth, migration, invasion, and induce epithelial to mesenchymal transition (EMT). The main signaling pathways mediating CR-1 effects include Nodal-dependent (Smad2/3) and Nodal-independent (Src/p44/42/Akt) signaling transduction pathways. In addition, there are several naturally occurring binding partner proteins (BPPs) for CR-1 that can either agonize or antagonize its bioactivity. We will review the collective role of CR-1 as an extracellular protein, discuss caveats to consider in developing a quantitation assay, define possible mechanistic avenues applicable for drug discovery, and report on our experimental approaches to overcome these problematic issues.",

keywords = "Autoantibodies, Cripto-1, Cripto-1 detection, drug discovery, inflammation",

author = "Malgorzata Klauzinska and Daniel Bertolette and Sudhamsh Tippireddy and Luigi Strizzi and Gray, {Peter C.} and Monica Gonzales and Meg Duroux and Menotti Ruvo and Christian Wechselberger and Castro, {Nadia P.} and Rangel, {Maria Cristina} and Annalia Foc{\`a} and Annamaria Sandomenico and Hendrix, {Mary J.C.} and David Salomon and Frank Cuttitta",

note = "Publisher Copyright: {\textcopyright} 2015 {\textcopyright} 2015 Taylor & Francis Group, LLC.",

year = "2015",

month = sep,

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doi = "10.3109/03008207.2015.1077239",

language = "English (US)",

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Klauzinska, M, Bertolette, D, Tippireddy, S, Strizzi, L, Gray, PC, Gonzales, M, Duroux, M, Ruvo, M, Wechselberger, C, Castro, NP, Rangel, MC, Focà, A, Sandomenico, A, Hendrix, MJC, Salomon, D & Cuttitta, F 2015, 'Cripto-1: An extracellular protein - Connecting the sequestered biological dots', Connective tissue research, vol. 56, no. 5, pp. 364-380. https://doi.org/10.3109/03008207.2015.1077239

TY - JOUR

T1 - Cripto-1

T2 - An extracellular protein - Connecting the sequestered biological dots

AU - Klauzinska, Malgorzata

AU - Bertolette, Daniel

AU - Tippireddy, Sudhamsh

AU - Strizzi, Luigi

AU - Gray, Peter C.

AU - Gonzales, Monica

AU - Duroux, Meg

AU - Ruvo, Menotti

AU - Wechselberger, Christian

AU - Castro, Nadia P.

AU - Rangel, Maria Cristina

AU - Focà, Annalia

AU - Sandomenico, Annamaria

AU - Hendrix, Mary J.C.

AU - Salomon, David

AU - Cuttitta, Frank

PY - 2015/9/3

Y1 - 2015/9/3

N2 - Cripto-1 (CR-1) is a multifunctional embryonic protein that is re-expressed during inflammation, wound repair, and malignant transformation. CR-1 can function either as a tethered co-receptor or shed as a free ligand underpinning its flexible role in cell physiology. CR-1 has been shown to mediate cell growth, migration, invasion, and induce epithelial to mesenchymal transition (EMT). The main signaling pathways mediating CR-1 effects include Nodal-dependent (Smad2/3) and Nodal-independent (Src/p44/42/Akt) signaling transduction pathways. In addition, there are several naturally occurring binding partner proteins (BPPs) for CR-1 that can either agonize or antagonize its bioactivity. We will review the collective role of CR-1 as an extracellular protein, discuss caveats to consider in developing a quantitation assay, define possible mechanistic avenues applicable for drug discovery, and report on our experimental approaches to overcome these problematic issues.

AB - Cripto-1 (CR-1) is a multifunctional embryonic protein that is re-expressed during inflammation, wound repair, and malignant transformation. CR-1 can function either as a tethered co-receptor or shed as a free ligand underpinning its flexible role in cell physiology. CR-1 has been shown to mediate cell growth, migration, invasion, and induce epithelial to mesenchymal transition (EMT). The main signaling pathways mediating CR-1 effects include Nodal-dependent (Smad2/3) and Nodal-independent (Src/p44/42/Akt) signaling transduction pathways. In addition, there are several naturally occurring binding partner proteins (BPPs) for CR-1 that can either agonize or antagonize its bioactivity. We will review the collective role of CR-1 as an extracellular protein, discuss caveats to consider in developing a quantitation assay, define possible mechanistic avenues applicable for drug discovery, and report on our experimental approaches to overcome these problematic issues.

KW - Autoantibodies

KW - Cripto-1

KW - Cripto-1 detection

KW - drug discovery

KW - inflammation

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JO - Connective tissue research

JF - Connective tissue research

IS - 5

ER -

Cripto-1: An extracellular protein - Connecting the sequestered biological dots

Abstract

Keywords

ASJC Scopus subject areas

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