Cross-Linked Micellar Spherical Nucleic Acids from Thermoresponsive Templates

Resham J. Banga, Brian Meckes, Suguna P. Narayan, Anthony J. Sprangers, Sonbinh T. Nguyen*, Chad A. Mirkin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

A one-pot synthesis of micellar spherical nucleic acid (SNA) nanostructures using Pluronic F127 as a thermoresponsive template is reported. These novel constructs are synthesized in a chemically straightforward process that involves intercalation of the lipid tails of DNA amphiphiles (CpG motifs for TLR-9 stimulation) into the hydrophobic regions of Pluronic F127 micelles, followed by chemical cross-linking and subsequent removal of non-cross-linked structures. The dense nucleic acid shell of the resulting cross-linked micellar SNA enhances their stability in physiological media and facilitates their rapid cellular internalization, making them effective TLR-9 immunomodulatory agents. These constructs underscore the potential of SNAs in regulating immune response and address the relative lack of stability of noncovalent constructs.

Original languageEnglish (US)
Pages (from-to)4278-4281
Number of pages4
JournalJournal of the American Chemical Society
Volume139
Issue number12
DOIs
StatePublished - Mar 29 2017

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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