Abstract
An antigen similar to a major protein of Mason-Pfizer monkey virus (MPMV) was found in breast cancers of 2 out of 9 women and in splenic tissue or peripheral leukocytes from 12 of 12 patients with myelogenous leukemias. Such antigen was not detected in other tumors or normal tissues. The cross-reactivity was detected using inhibition of the indirect quantitative radioimmunoprecipitation of 125I-labeled purified MPMV p25 and anti-MPMV antiserum. Particles banding in a density region of 1·15 to 1·17 g/ml, the density characteristic of the known animal leukemia viruses, were used as inhibitors for the precipitation. An antigen isolated from a spleen of a patient with myelogenous leukemia using anti-p25 immunoabsorbent column was similar in net electrical charge and molecular weight to MPMV p25. These results suggest that a virus-like particle bearing some antigenic relatedness to MPMV may be potentially important in the pathogenesis of human myelogenous leukemia.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 721-728 |
| Number of pages | 8 |
| Journal | European Journal of Cancer (1965) |
| Volume | 13 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 1977 |
Funding
TI~. MASOI~I--PF1:ZER monkey virus (MPMV), isolated from a spontaneous mammary carcinoma occurring in a Rhesus monkey \[1\], possesses morphological, biochemical and biophysical characteristics of known RNA tumor viruses \[2-4\]. The virus has been successfully propagated by cocultivation of the original mammary tumor with monkey embryo cells and by infection of chimpanzee and human cells \[5,6\]. MPMV-like particles have been identified in human cell lines derived from patients with leukemia or other malignancies \[7, 8, 9\] and in lines derived from normal cells which have spontaneously transformed \[10, 11\]. I report here that an antigen can be isolated from spleen cell.,~ of patients with myelogenous leukemia (ML). The antigen cross-reacts with and has very similar net electrical charge and molecular weight to MPMV p25. The results Accepted 30 Noveraber 1976. *This study was supported in part by a grant from the Leukemia Research Foundation and the National Cancer Institute, Grant NO1 CB 43998. ~'Present address : Department of Microbiology-Immunology, Northwestern University Medical Cen-ter~ Chicago, Illinois 60611, U.S.A.
ASJC Scopus subject areas
- General Medicine
