Crosslinking Strategies for 3D Bioprinting of Polymeric Hydrogels

Amin GhavamiNejad; Nureddin Ashammakhi; Xiao Yu Wu; Ali Khademhosseini

doi:10.1002/smll.202002931

Crosslinking Strategies for 3D Bioprinting of Polymeric Hydrogels

Amin GhavamiNejad^*, Nureddin Ashammakhi, Xiao Yu Wu, Ali Khademhosseini^*

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Review article › peer-review

282 Scopus citations

Abstract

Three-dimensional (3D) bioprinting has recently advanced as an important tool to produce viable constructs that can be used for regenerative purposes or as tissue models. To develop biomimetic and sustainable 3D constructs, several important processing aspects need to be considered, among which crosslinking is most important for achieving desirable biomechanical stability of printed structures, which is reflected in subsequent behavior and use of these constructs. In this work, crosslinking methods used in 3D bioprinting studies are reviewed, parameters that affect bioink chemistry are discussed, and the potential toward improving crosslinking outcomes and construct performance is highlighted. Furthermore, current challenges and future prospects are discussed. Due to the direct connection between crosslinking methods and properties of 3D bioprinted structures, this Review can provide a basis for developing necessary modifications to the design and manufacturing process of advanced tissue-like constructs in future.

Original language	English (US)
Article number	2002931
Journal	Small
Volume	16
Issue number	35
DOIs	https://doi.org/10.1002/smll.202002931
State	Published - Sep 1 2020

Funding

A.G. and N.A. contributed equally to this work. The authors acknowledge that they have no competing interests. The authors also acknowledge funding from the Natural Sciences and Engineering Research Council of Canada (Grant No. RGPIN-2019-07204) to X.Y.W. and National Institutes of Health (Grant Nos. EB021857, AR066193, AR057837, CA214411, HL137193, EB024403, EB023052, EB022403, and R01EB021857). A.G. and N.A. contributed equally to this work. The authors acknowledge that they have no competing interests. The authors also acknowledge funding from the Natural Sciences and Engineering Research Council of Canada (Grant No. RGPIN\u20102019\u201007204) to X.Y.W. and National Institutes of Health (Grant Nos. EB021857, AR066193, AR057837, CA214411, HL137193, EB024403, EB023052, EB022403, and R01EB021857).

Keywords

3D bioprinting
bioinks
crosslinking strategies
hydrogel–cell interactions
tissue engineering

ASJC Scopus subject areas

Biotechnology
General Chemistry
Biomaterials
General Materials Science
Engineering (miscellaneous)

Access to Document

10.1002/smll.202002931

Cite this

@article{4260de4d085c483782c5eecbff2ffad6,

title = "Crosslinking Strategies for 3D Bioprinting of Polymeric Hydrogels",

abstract = "Three-dimensional (3D) bioprinting has recently advanced as an important tool to produce viable constructs that can be used for regenerative purposes or as tissue models. To develop biomimetic and sustainable 3D constructs, several important processing aspects need to be considered, among which crosslinking is most important for achieving desirable biomechanical stability of printed structures, which is reflected in subsequent behavior and use of these constructs. In this work, crosslinking methods used in 3D bioprinting studies are reviewed, parameters that affect bioink chemistry are discussed, and the potential toward improving crosslinking outcomes and construct performance is highlighted. Furthermore, current challenges and future prospects are discussed. Due to the direct connection between crosslinking methods and properties of 3D bioprinted structures, this Review can provide a basis for developing necessary modifications to the design and manufacturing process of advanced tissue-like constructs in future.",

keywords = "3D bioprinting, bioinks, crosslinking strategies, hydrogel–cell interactions, tissue engineering",

author = "Amin GhavamiNejad and Nureddin Ashammakhi and Wu, {Xiao Yu} and Ali Khademhosseini",

note = "Funding Information: A.G. and N.A. contributed equally to this work. The authors acknowledge that they have no competing interests. The authors also acknowledge funding from the Natural Sciences and Engineering Research Council of Canada (Grant No. RGPIN‐2019‐07204) to X.Y.W. and National Institutes of Health (Grant Nos. EB021857, AR066193, AR057837, CA214411, HL137193, EB024403, EB023052, EB022403, and R01EB021857). Publisher Copyright: {\textcopyright} 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",

year = "2020",

month = sep,

day = "1",

doi = "10.1002/smll.202002931",

language = "English (US)",

volume = "16",

journal = "Small",

issn = "1613-6810",

publisher = "Wiley-VCH Verlag",

number = "35",

}

TY - JOUR

T1 - Crosslinking Strategies for 3D Bioprinting of Polymeric Hydrogels

AU - GhavamiNejad, Amin

AU - Ashammakhi, Nureddin

AU - Wu, Xiao Yu

AU - Khademhosseini, Ali

N1 - Funding Information: A.G. and N.A. contributed equally to this work. The authors acknowledge that they have no competing interests. The authors also acknowledge funding from the Natural Sciences and Engineering Research Council of Canada (Grant No. RGPIN‐2019‐07204) to X.Y.W. and National Institutes of Health (Grant Nos. EB021857, AR066193, AR057837, CA214411, HL137193, EB024403, EB023052, EB022403, and R01EB021857). Publisher Copyright: © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

PY - 2020/9/1

Y1 - 2020/9/1

N2 - Three-dimensional (3D) bioprinting has recently advanced as an important tool to produce viable constructs that can be used for regenerative purposes or as tissue models. To develop biomimetic and sustainable 3D constructs, several important processing aspects need to be considered, among which crosslinking is most important for achieving desirable biomechanical stability of printed structures, which is reflected in subsequent behavior and use of these constructs. In this work, crosslinking methods used in 3D bioprinting studies are reviewed, parameters that affect bioink chemistry are discussed, and the potential toward improving crosslinking outcomes and construct performance is highlighted. Furthermore, current challenges and future prospects are discussed. Due to the direct connection between crosslinking methods and properties of 3D bioprinted structures, this Review can provide a basis for developing necessary modifications to the design and manufacturing process of advanced tissue-like constructs in future.

AB - Three-dimensional (3D) bioprinting has recently advanced as an important tool to produce viable constructs that can be used for regenerative purposes or as tissue models. To develop biomimetic and sustainable 3D constructs, several important processing aspects need to be considered, among which crosslinking is most important for achieving desirable biomechanical stability of printed structures, which is reflected in subsequent behavior and use of these constructs. In this work, crosslinking methods used in 3D bioprinting studies are reviewed, parameters that affect bioink chemistry are discussed, and the potential toward improving crosslinking outcomes and construct performance is highlighted. Furthermore, current challenges and future prospects are discussed. Due to the direct connection between crosslinking methods and properties of 3D bioprinted structures, this Review can provide a basis for developing necessary modifications to the design and manufacturing process of advanced tissue-like constructs in future.

KW - 3D bioprinting

KW - bioinks

KW - crosslinking strategies

KW - hydrogel–cell interactions

KW - tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=85088802070&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85088802070&partnerID=8YFLogxK

U2 - 10.1002/smll.202002931

DO - 10.1002/smll.202002931

M3 - Review article

C2 - 32734720

AN - SCOPUS:85088802070

SN - 1613-6810

VL - 16

JO - Small

JF - Small

IS - 35

M1 - 2002931

ER -

Crosslinking Strategies for 3D Bioprinting of Polymeric Hydrogels

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this