Cryo-EM in the study of challenging systems: the human transcription pre-initiation complex

Eva Nogales, Robert K. Louder, Yuan He

Research output: Contribution to journalReview articlepeer-review

19 Scopus citations


Single particle cryo-Electron Microscopy (cryo-EM) is a technique that allows the structural characterization of macromolecules without the need for crystallization. For certain type of samples that are ideally suited for cryo-EM studies it has been possible to reach high-resolution structures following relatively standard procedures. Other biological systems remain highly challenging, even for cryo-EM. Challenges may involve the scarcity of the sample, poor stability of the complexes, and most often, the intrinsic flexibility of biological molecules. Among these challenging samples are large eukaryotic transcription complexes, which suffer from all such shortcomings. Here we report how we have recently tried to overcome those challenges in order to improve our structural understanding of the human transcription pre-initiation complex assembly and the transcription initiation process. Parallel efforts have also been carried out for budding yeast transcription initiation complexes, allowing comparisons that establish both the overall conservation and the specific structural differences between the two systems.

Original languageEnglish (US)
Pages (from-to)120-127
Number of pages8
JournalCurrent Opinion in Structural Biology
StatePublished - Oct 1 2016

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology


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