Cryoembedding and sectioning of cochleas for immunocytochemistry and in situ hybridization

Donna S Whitlon, Renee Szakaly, Mary A. Greiner

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

Current emphasis on biochemical and molecular aspects of cochlear anatomy underscores the necessity for high quality cryostat sections of the inner ear. The large volume of fluid space within the cochlea makes cryoembedding and sectioning of the organ more problematic than that of other, more homogeneous tissues. Our method for cryoembedding of cochleas for immunocytochemistry and in situ hybridization uses slow infiltration with increasing concentrations of sucrose followed by degassed embedding medium before final orientation and freezing. This method permits high quality cryosections to be cut which preserve overall structure and cellular resolution.

Original languageEnglish (US)
Pages (from-to)159-166
Number of pages8
JournalBrain Research Protocols
Volume6
Issue number3
DOIs
StatePublished - Jan 1 2001

Keywords

  • Cochlea
  • Cryosections
  • Hair cell
  • Immunocytochemistry
  • In situ hybridization
  • Mouse

ASJC Scopus subject areas

  • Neuroscience(all)

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