Abstract
Current emphasis on biochemical and molecular aspects of cochlear anatomy underscores the necessity for high quality cryostat sections of the inner ear. The large volume of fluid space within the cochlea makes cryoembedding and sectioning of the organ more problematic than that of other, more homogeneous tissues. Our method for cryoembedding of cochleas for immunocytochemistry and in situ hybridization uses slow infiltration with increasing concentrations of sucrose followed by degassed embedding medium before final orientation and freezing. This method permits high quality cryosections to be cut which preserve overall structure and cellular resolution.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 159-166 |
| Number of pages | 8 |
| Journal | Brain Research Protocols |
| Volume | 6 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jan 1 2001 |
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Keywords
- Cochlea
- Cryosections
- Hair cell
- Immunocytochemistry
- In situ hybridization
- Mouse
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
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Cryoembedding and sectioning of cochleas for immunocytochemistry and in situ hybridization. / Whitlon, Donna S; Szakaly, Renee; Greiner, Mary A.
In: Brain Research Protocols, Vol. 6, No. 3, 01.01.2001, p. 159-166.Research output: Contribution to journal › Article
TY - JOUR
T1 - Cryoembedding and sectioning of cochleas for immunocytochemistry and in situ hybridization
AU - Whitlon, Donna S
AU - Szakaly, Renee
AU - Greiner, Mary A.
PY - 2001/1/1
Y1 - 2001/1/1
N2 - Current emphasis on biochemical and molecular aspects of cochlear anatomy underscores the necessity for high quality cryostat sections of the inner ear. The large volume of fluid space within the cochlea makes cryoembedding and sectioning of the organ more problematic than that of other, more homogeneous tissues. Our method for cryoembedding of cochleas for immunocytochemistry and in situ hybridization uses slow infiltration with increasing concentrations of sucrose followed by degassed embedding medium before final orientation and freezing. This method permits high quality cryosections to be cut which preserve overall structure and cellular resolution.
AB - Current emphasis on biochemical and molecular aspects of cochlear anatomy underscores the necessity for high quality cryostat sections of the inner ear. The large volume of fluid space within the cochlea makes cryoembedding and sectioning of the organ more problematic than that of other, more homogeneous tissues. Our method for cryoembedding of cochleas for immunocytochemistry and in situ hybridization uses slow infiltration with increasing concentrations of sucrose followed by degassed embedding medium before final orientation and freezing. This method permits high quality cryosections to be cut which preserve overall structure and cellular resolution.
KW - Cochlea
KW - Cryosections
KW - Hair cell
KW - Immunocytochemistry
KW - In situ hybridization
KW - Mouse
UR - http://www.scopus.com/inward/record.url?scp=0034470033&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034470033&partnerID=8YFLogxK
U2 - 10.1016/S1385-299X(00)00048-9
DO - 10.1016/S1385-299X(00)00048-9
M3 - Article
VL - 6
SP - 159
EP - 166
JO - Brain Research Protocols
JF - Brain Research Protocols
SN - 1385-299X
IS - 3
ER -