Crystal structure determination of a neutral neurotoxin BmK M4 from Buthus martensii Karsch at 0.20 nm

Xiaolin He, Xinqi Liu, Zonghao Zeng, Hongmin Li, Miao Wang, Ying Zhang, Dacheng Wang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

BmK M4 is a neutral neurotoxin in the BmK toxin series. It is medially toxic and belongs to group III α-toxins. The purified sample was crystallized in rhombic space group P61. Using an X-ray diffraction technique, the crystal structure of BmK M4 was revealed by molecular replacement at 0.20 nm resolution. The model was refined. The final crystallographic R factor was 0.142 and the free R factor was 0.173. The root mean square deviation is 0.001 5 nm for the bond length and 1.753° for the bond angles. 64 water molecules were added to the asymmetric unit. The refined structure showed an unusual non-prolyl cis peptide bond at residue 10. The structure was compared with group II α-toxin BmK M8 (an acidic, weak toxin). The potential structural implications of the cis peptide bond were discussed.

Original languageEnglish (US)
Pages (from-to)45-46
Number of pages2
JournalScience in China, Series C: Life Sciences
Volume43
Issue number1
StatePublished - 2000

Keywords

  • Cis peptide bond
  • Crystal structure
  • Neutral toxin molecule
  • Scorpion neurotoxin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

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