Abstract
The crystal structure of the signal sequence binding subunit of the signal recognition particle (SRP) from Thermus aquaticus reveals a deep groove bounded by a flexible loop and lined with side chains of conserved hydrophobic residues. The groove defines a flexible, hydrophobic environment that is likely to contribute to the structural plasticity necessary for SRP to bind signal sequences of different lengths and amino acid sequence. The structure also reveals a helix-turn-helix motif containing an arginine-rich α helix that is required for binding to SRP RNA and is implicated in forming the core of an extended RNA binding surface.
Original language | English (US) |
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Pages (from-to) | 181-191 |
Number of pages | 11 |
Journal | Cell |
Volume | 94 |
Issue number | 2 |
DOIs | |
State | Published - Jul 24 1998 |
Funding
We thank P. Foster, E. Rutenber, and the staff at the Stanford Synchrotron Radiation Laboratory and the Cornell High Energy Synchrotron Source for assistance with data collection; R. Morse, P. Focia, S. LaPorte, and M. Butte for computational assistance; A. Shiau, A. Derman, U. Schmitz, C. Reyes, P. Peluso, and T. Powers for discussions; A. Frankel and R. Kelly for comments on the manuscript; and members of the Stroud, Walter, and Agard laboratories for advice provided at all stages. We also thank Tom Steitz for pointing out the structural similarity of the M domain to the HTH family of DNA-binding proteins. This work was supported by National Institutes of Health (NIH) Institutional Training Grant CA-09270 to R. J. K., by fellowships from the Herb Boyer Fund and the Biotechnology Program of the University of California to D. M. F., by NIH grant GM-24485 to R. M. S., and by NIH grant GM-32384 to P. W; P. W. is an Investigator of the Howard Hughes Medical Institute.
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology
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THE SIGNAL SEQUENCE BINDING PROTEIN FFH FROM THERMUS AQUATICUS
Keenan, R. J. (Contributor), Freymann, D. M. (Contributor), Walter, P. (Contributor) & Stroud, R. M. (Contributor), Protein Data Bank (PDB), Jul 16 1999
DOI: 10.2210/pdb2FFH/pdb, https://www.wwpdb.org/pdb?id=pdb_00002ffh
Dataset