Abstract
Staphylococcal leukotoxins are a family of β-barrel, bicomponent, pore-forming toxins with membrane-damaging functions. These bacterial exotoxins share sequence and structural homology and target several host-cell types. Leukotoxin ED (LukED) is one of these bicomponent pore-forming toxins that Staphylococcus aureus produces in order to suppress the ability of the host to contain the infection. The recent delineation of the important role that LukED plays in S. aureus pathogenesis and the identification of its protein receptors, combined with its presence in S. aureus methicillin-resistant epidemic strains, establish this leukocidin as a possible target for the development of novel therapeutics. Here, the crystal structures of the water-soluble LukE and LukD components of LukED have been determined. The two structures illustrate the tertiary-structural variability with respect to the other leukotoxins while retaining the conservation of the residues involved in the interaction of the protomers in the bipartite leukotoxin in the pore complex.
Original language | English (US) |
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Pages (from-to) | 113-120 |
Number of pages | 8 |
Journal | Acta Crystallographica Section D: Structural Biology |
Volume | 72 |
Issue number | 1 |
DOIs | |
State | Published - 2016 |
Funding
This project has been funded in whole or in part with Federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services under Contracts No. HHSN272200700058C and HHSN272201200026C. X-ray data collection for crystal structures was performed at the LS-CAT beamlines 21-ID-G (PDB entry 3roh) and 21-ID-F (PDB entry 4q7g) at the Advanced Photon Source Science User Facility operated for the US Department of Energy (DOE) supported by the US DOE under Contract No. DE-AC02-06CH11357.
Keywords
- Leukotoxin
- LukD
- LukE
- Pore-forming toxins
- Staphylococcus aureus
ASJC Scopus subject areas
- Structural Biology
Fingerprint
Dive into the research topics of 'Crystal structures of the components of the Staphylococcus aureus leukotoxin ED'. Together they form a unique fingerprint.Datasets
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Crystal Structure of Leukotoxin (LukE) from Staphylococcus aureus subsp. aureus COL.
Nocadello, S. (Contributor), Minasov, G. (Contributor), Shuvalova, L. (Contributor), Dubrovska, I. (Contributor), Sabini, E. (Contributor), Bagnoli, F. (Contributor), Grandi, G. (Contributor) & Anderson, W. F. (Contributor), Protein Data Bank (PDB), May 4 2011
DOI: 10.2210/pdb3ROH/pdb, https://www.wwpdb.org/pdb?id=pdb_00003roh
Dataset
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1.7 Angstrom Crystal Structure of leukotoxin LukD from Staphylococcus aureus.
Nocadello, S. (Contributor), Minasov, G. (Contributor), Shuvalova, L. (Contributor), Dubrovska, I. (Contributor), Sabini, E. (Contributor), Bagnoli, F. (Contributor), Grandi, G. (Contributor) & Anderson, W. F. (Contributor), Protein Data Bank (PDB), May 7 2014
DOI: 10.2210/pdb4Q7G/pdb, https://www.wwpdb.org/pdb?id=pdb_00004q7g
Dataset