Crystal structures of the components of the Staphylococcus aureus leukotoxin ED

S. Nocadello, George Minasov, Ludmilla A Shuvalova, I. Dubrovska, E. Sabini, F. Bagnoli, G. Grandi, Wayne F Anderson*

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Staphylococcal leukotoxins are a family of β-barrel, bicomponent, pore-forming toxins with membrane-damaging functions. These bacterial exotoxins share sequence and structural homology and target several host-cell types. Leukotoxin ED (LukED) is one of these bicomponent pore-forming toxins that Staphylococcus aureus produces in order to suppress the ability of the host to contain the infection. The recent delineation of the important role that LukED plays in S. aureus pathogenesis and the identification of its protein receptors, combined with its presence in S. aureus methicillin-resistant epidemic strains, establish this leukocidin as a possible target for the development of novel therapeutics. Here, the crystal structures of the water-soluble LukE and LukD components of LukED have been determined. The two structures illustrate the tertiary-structural variability with respect to the other leukotoxins while retaining the conservation of the residues involved in the interaction of the protomers in the bipartite leukotoxin in the pore complex.

Original languageEnglish (US)
Pages (from-to)113-120
Number of pages8
JournalActa Crystallographica Section D: Structural Biology
Volume72
Issue number1
DOIs
StatePublished - Jan 1 2016

Fingerprint

Staphylococcus aureus
Leukocidins
Exotoxins
Protein Subunits
Methicillin-Resistant Staphylococcus aureus
Sequence Homology
leukotoxin
Membranes
Water
Infection
Proteins
Therapeutics

Keywords

  • Leukotoxin
  • LukD
  • LukE
  • Pore-forming toxins
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Structural Biology

Cite this

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title = "Crystal structures of the components of the Staphylococcus aureus leukotoxin ED",
abstract = "Staphylococcal leukotoxins are a family of β-barrel, bicomponent, pore-forming toxins with membrane-damaging functions. These bacterial exotoxins share sequence and structural homology and target several host-cell types. Leukotoxin ED (LukED) is one of these bicomponent pore-forming toxins that Staphylococcus aureus produces in order to suppress the ability of the host to contain the infection. The recent delineation of the important role that LukED plays in S. aureus pathogenesis and the identification of its protein receptors, combined with its presence in S. aureus methicillin-resistant epidemic strains, establish this leukocidin as a possible target for the development of novel therapeutics. Here, the crystal structures of the water-soluble LukE and LukD components of LukED have been determined. The two structures illustrate the tertiary-structural variability with respect to the other leukotoxins while retaining the conservation of the residues involved in the interaction of the protomers in the bipartite leukotoxin in the pore complex.",
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Crystal structures of the components of the Staphylococcus aureus leukotoxin ED. / Nocadello, S.; Minasov, George; Shuvalova, Ludmilla A; Dubrovska, I.; Sabini, E.; Bagnoli, F.; Grandi, G.; Anderson, Wayne F.

In: Acta Crystallographica Section D: Structural Biology, Vol. 72, No. 1, 01.01.2016, p. 113-120.

Research output: Contribution to journalArticle

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AU - Nocadello, S.

AU - Minasov, George

AU - Shuvalova, Ludmilla A

AU - Dubrovska, I.

AU - Sabini, E.

AU - Bagnoli, F.

AU - Grandi, G.

AU - Anderson, Wayne F

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