Crystallization and X-ray diffraction analysis of an all-RNA U39C mutant of the minimal hairpin ribozyme

Valerie Grum-Tokars, Michael Milovanovic, Joseph E. Wedekind*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

An all-RNA 64-mer hairpin ribozyme has been crystallized in a form suitable for X-ray diffraction analysis at 3.35 Å resolution. The construct contains the U39C substitution, which enhances catalytic efficiency and represents the minimal catalytic motif formed by intermolecular docking of two helix-loop-helix domains. The crystallization strategy and results are presented.

Original languageEnglish (US)
Pages (from-to)142-145
Number of pages4
JournalActa Crystallographica - Section D Biological Crystallography
Volume59
Issue number1
DOIs
StatePublished - Jan 1 2003

ASJC Scopus subject areas

  • Structural Biology

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