TY - JOUR
T1 - Crystallization and X-ray diffraction analysis of an all-RNA U39C mutant of the minimal hairpin ribozyme
AU - Grum-Tokars, Valerie
AU - Milovanovic, Michael
AU - Wedekind, Joseph E.
PY - 2003/1/1
Y1 - 2003/1/1
N2 - An all-RNA 64-mer hairpin ribozyme has been crystallized in a form suitable for X-ray diffraction analysis at 3.35 Å resolution. The construct contains the U39C substitution, which enhances catalytic efficiency and represents the minimal catalytic motif formed by intermolecular docking of two helix-loop-helix domains. The crystallization strategy and results are presented.
AB - An all-RNA 64-mer hairpin ribozyme has been crystallized in a form suitable for X-ray diffraction analysis at 3.35 Å resolution. The construct contains the U39C substitution, which enhances catalytic efficiency and represents the minimal catalytic motif formed by intermolecular docking of two helix-loop-helix domains. The crystallization strategy and results are presented.
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U2 - 10.1107/S0907444902019066
DO - 10.1107/S0907444902019066
M3 - Article
C2 - 12499551
AN - SCOPUS:0037243910
SN - 0907-4449
VL - 59
SP - 142
EP - 145
JO - Acta Crystallographica - Section D Biological Crystallography
JF - Acta Crystallographica - Section D Biological Crystallography
IS - 1
ER -