Crystallization of a complex of cro repressor with a 17 base-pair operator

Richard G. Brennan*, Yoshinori Takeda, Jim Kim, Wayne F. Anderson, Brian W. Matthews

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Crystals of the λ cro repressor complexed to a 17 base-pair synthetic binding site related to the OR3 operator have been obtained. The complex crystallizes in the hexagonal space group P62 (or P64) with unit cell dimensions a = b = 154.8 A ̊, c = 85.6 A ̊. Preliminary photography reveals that the crystals are stable to X-rays and display measurable reflections to a resolution of about 3.7 Å. The diffraction patterns suggest that the cro-DNA complexes are arranged in an open hexagonal network with the DNA fragments stacked end-to-end. The DNA is in the B-form but appears to be bent or curved into an approximate superhelix.

Original languageEnglish (US)
Pages (from-to)115-118
Number of pages4
JournalJournal of Molecular Biology
Volume188
Issue number1
DOIs
StatePublished - Mar 5 1986

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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