Crystallization of a complex of cro repressor with a 17 base-pair operator

Richard G. Brennan; Yoshinori Takeda; Jim Kim; Wayne F. Anderson; Brian W. Matthews

doi:10.1016/0022-2836(86)90488-2

Crystallization of a complex of cro repressor with a 17 base-pair operator

Richard G. Brennan^*, Yoshinori Takeda, Jim Kim, Wayne F. Anderson, Brian W. Matthews

^*Corresponding author for this work

Biochemistry and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Crystals of the λ cro repressor complexed to a 17 base-pair synthetic binding site related to the O_R3 operator have been obtained. The complex crystallizes in the hexagonal space group P6₂ (or P6₄) with unit cell dimensions a = b = 154.8 A ̊, c = 85.6 A ̊. Preliminary photography reveals that the crystals are stable to X-rays and display measurable reflections to a resolution of about 3.7 Å. The diffraction patterns suggest that the cro-DNA complexes are arranged in an open hexagonal network with the DNA fragments stacked end-to-end. The DNA is in the B-form but appears to be bent or curved into an approximate superhelix.

Original language	English (US)
Pages (from-to)	115-118
Number of pages	4
Journal	Journal of Molecular Biology
Volume	188
Issue number	1
DOIs	https://doi.org/10.1016/0022-2836(86)90488-2
State	Published - Mar 5 1986

Funding

LVr, thank I)r Aaron Klug for helpful discussionos n the tiiffraction from superhelicrs and also wish to acknowledge the advice and assistance of Dr Larry-\Yravrr. This work was supported by grants from the Sational Institutes of Health (GM20066) to R.W.M. and (CM30894) to Y.T., the National Science Foundation (l’(‘h18312151) to K.W.M. and by a,n NIH postdoctoral fellowship (GMl0476) to R,.G.R.

ASJC Scopus subject areas

Molecular Biology
Structural Biology

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10.1016/0022-2836(86)90488-2

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title = "Crystallization of a complex of cro repressor with a 17 base-pair operator",

abstract = "Crystals of the λ cro repressor complexed to a 17 base-pair synthetic binding site related to the OR3 operator have been obtained. The complex crystallizes in the hexagonal space group P62 (or P64) with unit cell dimensions a = b = 154.8 A ̊, c = 85.6 A ̊. Preliminary photography reveals that the crystals are stable to X-rays and display measurable reflections to a resolution of about 3.7 {\AA}. The diffraction patterns suggest that the cro-DNA complexes are arranged in an open hexagonal network with the DNA fragments stacked end-to-end. The DNA is in the B-form but appears to be bent or curved into an approximate superhelix.",

author = "Brennan, {Richard G.} and Yoshinori Takeda and Jim Kim and Anderson, {Wayne F.} and Matthews, {Brian W.}",

note = "Funding Information: LVr, thank I)r Aaron Klug for helpful discussionos n the tiiffraction from superhelicrs and also wish to acknowledge the advice and assistance of Dr Larry-\Yravrr. This work was supported by grants from the Sational Institutes of Health (GM20066) to R.W.M. and (CM30894) to Y.T., the National Science Foundation (l{\textquoteright}({\textquoteleft}h18312151) to K.W.M. and by a,n NIH postdoctoral fellowship (GMl0476) to R,.G.R.",

year = "1986",

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doi = "10.1016/0022-2836(86)90488-2",

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T1 - Crystallization of a complex of cro repressor with a 17 base-pair operator

AU - Brennan, Richard G.

AU - Takeda, Yoshinori

AU - Kim, Jim

AU - Anderson, Wayne F.

AU - Matthews, Brian W.

N1 - Funding Information: LVr, thank I)r Aaron Klug for helpful discussionos n the tiiffraction from superhelicrs and also wish to acknowledge the advice and assistance of Dr Larry-\Yravrr. This work was supported by grants from the Sational Institutes of Health (GM20066) to R.W.M. and (CM30894) to Y.T., the National Science Foundation (l’(‘h18312151) to K.W.M. and by a,n NIH postdoctoral fellowship (GMl0476) to R,.G.R.

PY - 1986/3/5

Y1 - 1986/3/5

N2 - Crystals of the λ cro repressor complexed to a 17 base-pair synthetic binding site related to the OR3 operator have been obtained. The complex crystallizes in the hexagonal space group P62 (or P64) with unit cell dimensions a = b = 154.8 A ̊, c = 85.6 A ̊. Preliminary photography reveals that the crystals are stable to X-rays and display measurable reflections to a resolution of about 3.7 Å. The diffraction patterns suggest that the cro-DNA complexes are arranged in an open hexagonal network with the DNA fragments stacked end-to-end. The DNA is in the B-form but appears to be bent or curved into an approximate superhelix.

AB - Crystals of the λ cro repressor complexed to a 17 base-pair synthetic binding site related to the OR3 operator have been obtained. The complex crystallizes in the hexagonal space group P62 (or P64) with unit cell dimensions a = b = 154.8 A ̊, c = 85.6 A ̊. Preliminary photography reveals that the crystals are stable to X-rays and display measurable reflections to a resolution of about 3.7 Å. The diffraction patterns suggest that the cro-DNA complexes are arranged in an open hexagonal network with the DNA fragments stacked end-to-end. The DNA is in the B-form but appears to be bent or curved into an approximate superhelix.

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JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 1

ER -

Crystallization of a complex of cro repressor with a 17 base-pair operator

Abstract

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