Crystals of the λ cro repressor complexed to a 17 base-pair synthetic binding site related to the OR3 operator have been obtained. The complex crystallizes in the hexagonal space group P62 (or P64) with unit cell dimensions a = b = 154.8 A ̊, c = 85.6 A ̊. Preliminary photography reveals that the crystals are stable to X-rays and display measurable reflections to a resolution of about 3.7 Å. The diffraction patterns suggest that the cro-DNA complexes are arranged in an open hexagonal network with the DNA fragments stacked end-to-end. The DNA is in the B-form but appears to be bent or curved into an approximate superhelix.
|Original language||English (US)|
|Number of pages||4|
|Journal||Journal of Molecular Biology|
|State||Published - Mar 5 1986|
ASJC Scopus subject areas
- Structural Biology
- Molecular Biology