TY - JOUR
T1 - Crystallization of soluble urokinase receptor (suPAR) in complex with urokinase amino-terminal fragment (1-143)
AU - Huang, Mingdong
AU - Mazar, Andrew P.
AU - Parry, Graham
AU - Higazi, Abd Al Roof
AU - Kuo, Alice
AU - Cines, Douglas B.
PY - 2005/6
Y1 - 2005/6
N2 - Urokinase-type plasminogen activator (urokinase, uPA) and its receptor, uPAR, have been implicated in cell adhesion, migration, tissue remodelling and tumour-cell invasion. uPAR has three domains and is anchored to membranes by a glycosyl-phosphatidylinositol (GPI) anchor. Recombinant uPAR without its GPI anchor, soluble uPAR (suPAR), tends to oligomerize, making it difficult to crystallize. The amino-terminal fragment (ATF) of uPA is the major receptor-binding determinant in suPAR and binds to suPAR with nanomolar affinity, indistinguishable from membrane-bound uPAR. It is shown that uPA is capable of dissociating the oligomerization of suPAR and the crystallization of the suPAR-ATF complex is reported here. The resulting crystals diffract to 3.1 Å using a synchrotron X-ray source.
AB - Urokinase-type plasminogen activator (urokinase, uPA) and its receptor, uPAR, have been implicated in cell adhesion, migration, tissue remodelling and tumour-cell invasion. uPAR has three domains and is anchored to membranes by a glycosyl-phosphatidylinositol (GPI) anchor. Recombinant uPAR without its GPI anchor, soluble uPAR (suPAR), tends to oligomerize, making it difficult to crystallize. The amino-terminal fragment (ATF) of uPA is the major receptor-binding determinant in suPAR and binds to suPAR with nanomolar affinity, indistinguishable from membrane-bound uPAR. It is shown that uPA is capable of dissociating the oligomerization of suPAR and the crystallization of the suPAR-ATF complex is reported here. The resulting crystals diffract to 3.1 Å using a synchrotron X-ray source.
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U2 - 10.1107/S0907444905014174
DO - 10.1107/S0907444905014174
M3 - Article
C2 - 15930623
AN - SCOPUS:23844471314
SN - 0907-4449
VL - 61
SP - 697
EP - 700
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 6
ER -