Crystallographic data for complexes of the Cro repressor with DNA

Wayne F. Anderson; Miroslaw Cygler; Margaret Vandonselaar; Douglas H. Ohlendorf; Brian W. Matthews; Jim Kim; Yoshinori Takeda

doi:10.1016/S0022-2836(83)80082-5

Crystallographic data for complexes of the Cro repressor with DNA

Wayne F. Anderson^*, Miroslaw Cygler, Margaret Vandonselaar, Douglas H. Ohlendorf, Brian W. Matthews, Jim Kim, Yoshinori Takeda

^*Corresponding author for this work

Biochemistry and Molecular Genetics

Research output: Contribution to journal › Letter › peer-review

14 Scopus citations

Abstract

Complexes of the bacteriophage λ Cro repressor with two DNA duplexes have been crystallized. The DNA sequences are equimolar mixtures of ApTpCpApCpC and its complementary strand and ApCpCpGpCpApApGpG and its complementary strand, which are both parts of the λ O_R3 operator. The space group of both co-crystals is C222₁ with cell dimensions a=81·1 Å, b=89·2 Å, and c=80·0 Å. Analysis of dissolved crystals shows that they respectively contain approximately two hexamers per Cro dimer and one nonamer per dimer. The cocrystals diffract to about 3 Å resolution and appear suitable for structural studies.

Original language	English (US)
Pages (from-to)	903-906
Number of pages	4
Journal	Journal of Molecular Biology
Volume	168
Issue number	4
DOIs	https://doi.org/10.1016/S0022-2836(83)80082-5
State	Published - Aug 25 1983

Funding

This work was supported by the Canadian Medical Research Council through the MRC Group on Protein Structure and Function and by grants from the National Institutes of Health (GM20066, GM28138, GM30894) and the National Science Foundation (PCM-8014311) and an NIH postdoctoral fellowship to one of the authors (D.H.O.).

ASJC Scopus subject areas

Molecular Biology
Structural Biology

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@article{1e7d240562eb4a2bb40b6a8324844ecc,

title = "Crystallographic data for complexes of the Cro repressor with DNA",

abstract = "Complexes of the bacteriophage λ Cro repressor with two DNA duplexes have been crystallized. The DNA sequences are equimolar mixtures of ApTpCpApCpC and its complementary strand and ApCpCpGpCpApApGpG and its complementary strand, which are both parts of the λ OR3 operator. The space group of both co-crystals is C2221 with cell dimensions a=81·1 {\AA}, b=89·2 {\AA}, and c=80·0 {\AA}. Analysis of dissolved crystals shows that they respectively contain approximately two hexamers per Cro dimer and one nonamer per dimer. The cocrystals diffract to about 3 {\AA} resolution and appear suitable for structural studies.",

author = "Anderson, {Wayne F.} and Miroslaw Cygler and Margaret Vandonselaar and Ohlendorf, {Douglas H.} and Matthews, {Brian W.} and Jim Kim and Yoshinori Takeda",

note = "Funding Information: This work was supported by the Canadian Medical Research Council through the MRC Group on Protein Structure and Function and by grants from the National Institutes of Health (GM20066, GM28138, GM30894) and the National Science Foundation (PCM-8014311) and an NIH postdoctoral fellowship to one of the authors (D.H.O.).",

year = "1983",

month = aug,

day = "25",

doi = "10.1016/S0022-2836(83)80082-5",

language = "English (US)",

volume = "168",

pages = "903--906",

journal = "Journal of Molecular Biology",

issn = "0022-2836",

publisher = "Academic Press Inc.",

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TY - JOUR

T1 - Crystallographic data for complexes of the Cro repressor with DNA

AU - Anderson, Wayne F.

AU - Cygler, Miroslaw

AU - Vandonselaar, Margaret

AU - Ohlendorf, Douglas H.

AU - Matthews, Brian W.

AU - Kim, Jim

AU - Takeda, Yoshinori

N1 - Funding Information: This work was supported by the Canadian Medical Research Council through the MRC Group on Protein Structure and Function and by grants from the National Institutes of Health (GM20066, GM28138, GM30894) and the National Science Foundation (PCM-8014311) and an NIH postdoctoral fellowship to one of the authors (D.H.O.).

PY - 1983/8/25

Y1 - 1983/8/25

N2 - Complexes of the bacteriophage λ Cro repressor with two DNA duplexes have been crystallized. The DNA sequences are equimolar mixtures of ApTpCpApCpC and its complementary strand and ApCpCpGpCpApApGpG and its complementary strand, which are both parts of the λ OR3 operator. The space group of both co-crystals is C2221 with cell dimensions a=81·1 Å, b=89·2 Å, and c=80·0 Å. Analysis of dissolved crystals shows that they respectively contain approximately two hexamers per Cro dimer and one nonamer per dimer. The cocrystals diffract to about 3 Å resolution and appear suitable for structural studies.

AB - Complexes of the bacteriophage λ Cro repressor with two DNA duplexes have been crystallized. The DNA sequences are equimolar mixtures of ApTpCpApCpC and its complementary strand and ApCpCpGpCpApApGpG and its complementary strand, which are both parts of the λ OR3 operator. The space group of both co-crystals is C2221 with cell dimensions a=81·1 Å, b=89·2 Å, and c=80·0 Å. Analysis of dissolved crystals shows that they respectively contain approximately two hexamers per Cro dimer and one nonamer per dimer. The cocrystals diffract to about 3 Å resolution and appear suitable for structural studies.

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DO - 10.1016/S0022-2836(83)80082-5

M3 - Letter

C2 - 6887256

AN - SCOPUS:0021112485

SN - 0022-2836

VL - 168

SP - 903

EP - 906

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 4

ER -

Crystallographic data for complexes of the Cro repressor with DNA

Abstract

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ASJC Scopus subject areas

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