Crystallographic data for complexes of the Cro repressor with DNA

Wayne F. Anderson*, Miroslaw Cygler, Margaret Vandonselaar, Douglas H. Ohlendorf, Brian W. Matthews, Jim Kim, Yoshinori Takeda

*Corresponding author for this work

Research output: Contribution to journalLetterpeer-review

14 Scopus citations

Abstract

Complexes of the bacteriophage λ Cro repressor with two DNA duplexes have been crystallized. The DNA sequences are equimolar mixtures of ApTpCpApCpC and its complementary strand and ApCpCpGpCpApApGpG and its complementary strand, which are both parts of the λ OR3 operator. The space group of both co-crystals is C2221 with cell dimensions a=81·1 Å, b=89·2 Å, and c=80·0 Å. Analysis of dissolved crystals shows that they respectively contain approximately two hexamers per Cro dimer and one nonamer per dimer. The cocrystals diffract to about 3 Å resolution and appear suitable for structural studies.

Original languageEnglish (US)
Pages (from-to)903-906
Number of pages4
JournalJournal of Molecular Biology
Volume168
Issue number4
DOIs
StatePublished - Aug 25 1983

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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