Crystallographic texture and elemental composition mapped in bovine root dentin at the 200-nm level

A. C. Deymier-Black, A. Veis, Z. Cai, S. R. Stock*

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The relationship between the mineralization of peritubular dentin (PTD) and intertubular dentin (ITD) is not well understood. Tubules are quite small, diameter â̂2-μm, and this makes the near-tubule region of dentin difficult to study. Here, advanced characterization techniques are applied in a novel way to examine what organic or nanostructural signatures may indicate the end of ITD or the beginning of PTD mineralization. X-ray fluorescence intensity (Ca, P, and Zn) and X-ray diffraction patterns from carbonated apatite (cAp) were mapped around dentintubules at resolutions ten times smaller than the feature size (200-nm pixels), representing a 36% increase in resolution over earlier work. In the near tubule volumes of near-pulp, root dentin, Zn intensity was higher than in ITD remote from the tubules. This increase in Zn 2+, as determined by X-ray absorption near edge structure analysis, may indicate the presence of metalloenzymes or transcription factors important to ITD or PTD mineralization. The profiles of the cAp 00.2 X-ray diffraction rings were fitted with a pseudo-Voigt function, and the spatial and azimuthal distribution of these rings' integrated intensities indicated that the cAp platelets were arranged with their c-axes aligned tangential to the edge of the tubule lumen. This texture was continuous throughout the dentin indicating a lack of structural difference between in the Zn rich near-tubular region and the remote ITD.

Original languageEnglish (US)
Pages (from-to)231-240
Number of pages10
JournalScanning
Volume36
Issue number2
DOIs
StatePublished - Jan 1 2014

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Keywords

  • X-ray diffraction mapping
  • X-ray fluorescence mapping
  • dentin
  • tubule
  • zinc

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Instrumentation

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