CueR activates transcription through a DNA distortion mechanism

Chengli Fang, Steven J. Philips, Xiaoxian Wu, Kui Chen, Jing Shi, Liqiang Shen, Juncao Xu, Yu Feng, Thomas V. O’Halloran, Yu Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


The MerR-family transcription factors (TFs) are a large group of bacterial proteins responding to cellular metal ions and multiple antibiotics by binding within central RNA polymerase-binding regions of a promoter. While most TFs alter transcription through protein–protein interactions, MerR TFs are capable of reshaping promoter DNA. To address the question of which mechanism prevails, we determined two cryo-EM structures of transcription activation complexes (TAC) comprising Escherichia coli CueR (a prototype MerR TF), RNAP holoenzyme and promoter DNA. The structures reveal that this TF promotes productive promoter–polymerase association without canonical protein–protein contacts seen between other activator proteins and RNAP. Instead, CueR realigns the key promoter elements in the transcription activation complex by clamp-like protein–DNA interactions: these induce four distinct kinks that ultimately position the −10 element for formation of the transcription bubble. These structural and biochemical results provide strong support for the DNA distortion paradigm of allosteric transcriptional control by MerR TFs. [Figure not available: see fulltext.].

Original languageEnglish (US)
Pages (from-to)57-64
Number of pages8
JournalNature Chemical Biology
Issue number1
StatePublished - Jan 2021

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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