Culture of human mast cells from peripheral blood progenitors

Hirohisa Saito; Atsushi Kato; Kenji Matsumoto; Yoshimichi Okayama

doi:10.1038/nprot.2006.344

Culture of human mast cells from peripheral blood progenitors

Hirohisa Saito^*, Atsushi Kato, Kenji Matsumoto, Yoshimichi Okayama

^*Corresponding author for this work

Medicine, Allergy Division

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

This protocol details a method for obtaining a considerable number of human mast cells from peripheral blood cells from normal donors without using stem cell mobilization treatment. By using the magnetic cell sorting system, 10⁴-10⁵ cells are retrieved in the CD34⁺ fraction when 100 ml of blood is drawn from a healthy donor. When these cells are cultured using methylcellulose medium supplemented with stem cell factor and interleukin 6, 10³-10⁴ mast cell colonies are formed in 6 weeks. The total mast cell number at 6 weeks of culture will be 10⁶-10⁷.

Original language	English (US)
Pages (from-to)	2178-2183
Number of pages	6
Journal	Nature Protocols
Volume	1
Issue number	4
DOIs	https://doi.org/10.1038/nprot.2006.344
State	Published - Nov 2006

Funding

ACKNOWLEDGMENTS We thank Mr. Takahisa Ogasawara and Mr. Keisuke Yuki for their help with the experimental details. This work was supported by Grant ID05-24 from the National Institute of Biomedical Innovation.

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1038/nprot.2006.344

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title = "Culture of human mast cells from peripheral blood progenitors",

abstract = "This protocol details a method for obtaining a considerable number of human mast cells from peripheral blood cells from normal donors without using stem cell mobilization treatment. By using the magnetic cell sorting system, 104-105 cells are retrieved in the CD34+ fraction when 100 ml of blood is drawn from a healthy donor. When these cells are cultured using methylcellulose medium supplemented with stem cell factor and interleukin 6, 103-104 mast cell colonies are formed in 6 weeks. The total mast cell number at 6 weeks of culture will be 106-107.",

author = "Hirohisa Saito and Atsushi Kato and Kenji Matsumoto and Yoshimichi Okayama",

note = "Funding Information: ACKNOWLEDGMENTS We thank Mr. Takahisa Ogasawara and Mr. Keisuke Yuki for their help with the experimental details. This work was supported by Grant ID05-24 from the National Institute of Biomedical Innovation.",

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AU - Saito, Hirohisa

AU - Kato, Atsushi

AU - Matsumoto, Kenji

AU - Okayama, Yoshimichi

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N2 - This protocol details a method for obtaining a considerable number of human mast cells from peripheral blood cells from normal donors without using stem cell mobilization treatment. By using the magnetic cell sorting system, 104-105 cells are retrieved in the CD34+ fraction when 100 ml of blood is drawn from a healthy donor. When these cells are cultured using methylcellulose medium supplemented with stem cell factor and interleukin 6, 103-104 mast cell colonies are formed in 6 weeks. The total mast cell number at 6 weeks of culture will be 106-107.

AB - This protocol details a method for obtaining a considerable number of human mast cells from peripheral blood cells from normal donors without using stem cell mobilization treatment. By using the magnetic cell sorting system, 104-105 cells are retrieved in the CD34+ fraction when 100 ml of blood is drawn from a healthy donor. When these cells are cultured using methylcellulose medium supplemented with stem cell factor and interleukin 6, 103-104 mast cell colonies are formed in 6 weeks. The total mast cell number at 6 weeks of culture will be 106-107.

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Culture of human mast cells from peripheral blood progenitors

Abstract

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ASJC Scopus subject areas

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