Culture of human mast cells from peripheral blood progenitors

Hirohisa Saito*, Atsushi Kato, Kenji Matsumoto, Yoshimichi Okayama

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

This protocol details a method for obtaining a considerable number of human mast cells from peripheral blood cells from normal donors without using stem cell mobilization treatment. By using the magnetic cell sorting system, 104-105 cells are retrieved in the CD34+ fraction when 100 ml of blood is drawn from a healthy donor. When these cells are cultured using methylcellulose medium supplemented with stem cell factor and interleukin 6, 103-104 mast cell colonies are formed in 6 weeks. The total mast cell number at 6 weeks of culture will be 106-107.

Original languageEnglish (US)
Pages (from-to)2178-2183
Number of pages6
JournalNature Protocols
Volume1
Issue number4
DOIs
StatePublished - Nov 2006

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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