This protocol details a method for obtaining a considerable number of human mast cells from peripheral blood cells from normal donors without using stem cell mobilization treatment. By using the magnetic cell sorting system, 104-105 cells are retrieved in the CD34+ fraction when 100 ml of blood is drawn from a healthy donor. When these cells are cultured using methylcellulose medium supplemented with stem cell factor and interleukin 6, 103-104 mast cell colonies are formed in 6 weeks. The total mast cell number at 6 weeks of culture will be 106-107.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)