Culture pH affects expression rates and glycosylation of recombinant mouse placental lactogen proteins by chinese hamster ovary (CHO) cells

Michael C. Borys, Daniel I.H. Linzer, Eleftherios T. Papoutsakis*

*Corresponding author for this work

Research output: Contribution to journalArticle

121 Scopus citations

Abstract

Glycosylation patterns and specific expression rates of the recombinant protein mouse placental lactogen-I (mPL-I) by Chinese hamster ovary (CHO) cells varied significantly over the extracellular pH (pHe) range of 6.1 to 8.7. The maximum specific mPL-I expression rates occurred between pHe 7.6 and 8.0. The pHe effect on protein expression was confirmed using a different CHO cell expressing the unglycosylated recombinant protein mouse placental lactogen-II (mPL-II). Decreases in the extent of glycosylation of mPL-I were observed at low (below 6.9) and high (above 8.2) pHe values. The pHe dependent variations in mPL-I accumulation in the supernatant as well as in glycosylation patterns were not the result of enzymatic degradation in the culture medium.

Original languageEnglish (US)
Pages (from-to)720-724
Number of pages5
JournalBio/Technology
Volume11
Issue number6
DOIs
StatePublished - Jun 1993

ASJC Scopus subject areas

  • Biotechnology

Fingerprint Dive into the research topics of 'Culture pH affects expression rates and glycosylation of recombinant mouse placental lactogen proteins by chinese hamster ovary (CHO) cells'. Together they form a unique fingerprint.

Cite this