Curvature-Induced base pair "Slipping" Effects in dna-nanoparticle hybridization

Haley D. Hill, Sarah J. Hurst, Chad A. Mirkin

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Experiments are presented that suggest DNA strands chemically immobilized on gold nanoparticle surfaces can engage in two types of hybridization: one that involves complementary strands and normal base pairing interactions and a second one assigned as a "slipping" interaction, which can additionally stabilize the aggregate structures through non-Watson-Crick type base pairing or interactions less complementary than the primary interaction. The curvature of the particles appears to be a major factor that contributes to the formation of these slipping interactions as evidenced by the observation that flat gold triangular nanoprism conjugates of the same sequence do not support them. Finally, these slipping interactions significantly stabilize nanoparticle aggregate structures, leading to large increases in T m's and effective association constants as compared with free DNA and particles that do not have the appropriate sequence to maximize their contribution.

Original languageEnglish (US)
Pages (from-to)317-321
Number of pages5
JournalNano letters
Volume9
Issue number1
DOIs
StatePublished - Jan 2009

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Fingerprint Dive into the research topics of 'Curvature-Induced base pair "Slipping" Effects in dna-nanoparticle hybridization'. Together they form a unique fingerprint.

Cite this