Cutting edge: IL-4 production by mast cells does not require c-maf

Melanie A. Sherman, Tammy Y. Nachman, Melissa A. Brown*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The c-maf transcription factor is selectively expressed in IL-4- producing Th2 cells. It has been implicated in IL-4 gene transcription based on its ability to directly activate the IL-4 gene in nonexpressing B cells and to promote IL-4-induced Th2 differentiation. However, it has not been definitively shown that IL-4 production by other cells is dependent on the presence of c-maf. Here, we show that IL-4-producing mast cells do not express the c-maf factor. Furthermore, mutation of a defined c-maf binding site within the proximal IL-4 promoter, which profoundly affects transcription in T cells, has no effect on expression of a reporter gene driven by the IL-4 promoter in mast cells. These results demonstrate that c- maf and its target binding site are not required for IL-4 production in all cell types and delineate additional cis- and trans-acting elements that contribute to the cell-type specific transcriptional regulation of IL-4.

Original languageEnglish (US)
Pages (from-to)1733-1736
Number of pages4
JournalJournal of Immunology
Volume163
Issue number4
StatePublished - Aug 15 1999

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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