CXCR4 signaling regulates radial glial morphology and cell fate during embryonic spinal cord development

Divakar S. Mithal, Dongjun Ren, Richard J. Miller*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Embryonic meninges secrete the chemokine SDF-1/CXCL12 as a chemotactic guide for migrating neural stem cells, but SDF-1 is not known to directly regulate the functions of radial glia. Recently, the developing meninges have been shown to regulate radial glial function, yet the mechanisms and signals responsible for this phenomenon remain unclear. Moreover, as a nonmigratory cell type, radial glia do not conform to traditional models associated with chemokine signaling in the central nervous system. Using fluorescent transgenes, in vivo genetic manipulations and pharmacological techniques, we demonstrate that SDF-1 derived from the meninges exerts a CXCR4-dependent effect on radial glia. Deletion of CXCR4 expression by radial glia influences their morphology, mitosis, and progression through both oligodendroglial and astroglial lineages. Additionally, disruption of CXCR4 signaling in radial glia has a transient effect on the migration of oligodendrocyte progenitors. These data indicate that a specific chemokine signal derived from the meninges has multiple regulatory effects on radial glia.

Original languageEnglish (US)
Pages (from-to)1288-1305
Number of pages18
JournalGlia
Volume61
Issue number8
DOIs
StatePublished - Aug 2013

Keywords

  • CXCR4
  • Chemokine receptor
  • Oligodendrocyte progenitor
  • Radial glia
  • SDF-1

ASJC Scopus subject areas

  • Neurology
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'CXCR4 signaling regulates radial glial morphology and cell fate during embryonic spinal cord development'. Together they form a unique fingerprint.

Cite this