Cyclin E overexpression responsible for growth of human hepatic tumors with p21(WAF1/CIP1/SDI1)

Toshiya Tsuji, Masahiro Miyazaki, Kazuo Fushimi, Koichiro Mihara, Yusuke Inoue, Ryuichiro Ohashi, Motoaki Ohtsubo, Keisuke Hamazaki, Shouji Furusako, Masayoshi Namba*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


We examined a relationship between p21(WAF1/CIP1/SDI1) and cell-cycle-related proteins in 12 human liver tumor cell lines (JHH-1, -2, -4, -5, -6, -7; HLE; HuH-7; Hep3B; PLC/PRF/5; HuH-6; HepG2). Seven (JHH-1, -2, -5, -6, -7; Hep3B; HepG2) out of eight cell lines having p21(WAF1/CIP1/SDI1) protein overexpressed cyclin E protein, although one of them (JHH-5) overexpressed a reduced size of cyclin E. The rest (HuH-6) of the 8 cell lines with p21(WAF1/CIP1/SDI1) showed a decreased expression of cyclin E. Four cell lines (JHH-4; HLE; HuH-7; PLC/PRF/5) deficient of p21(WAF1/CIP1/SDI1) protein did not overexpress cyclin E protein. As to expression of the other cell-cycle-related proteins, cyclin A, cyclin D1, CDK2 or CDK4, no significant difference was detected among the 12 cell lines. These findings indicate that the human liver tumor cell lines which have the p21(WAF1/CIP1/SDI1)-inducible barriers of the cell cycle progression can go through the G1/S checkpoint by overexpressing cyclin E.

Original languageEnglish (US)
Pages (from-to)317-321
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - Jan 14 1998

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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