TY - JOUR
T1 - Cyclin E overexpression responsible for growth of human hepatic tumors with p21(WAF1/CIP1/SDI1)
AU - Tsuji, Toshiya
AU - Miyazaki, Masahiro
AU - Fushimi, Kazuo
AU - Mihara, Koichiro
AU - Inoue, Yusuke
AU - Ohashi, Ryuichiro
AU - Ohtsubo, Motoaki
AU - Hamazaki, Keisuke
AU - Furusako, Shouji
AU - Namba, Masayoshi
PY - 1998/1/14
Y1 - 1998/1/14
N2 - We examined a relationship between p21(WAF1/CIP1/SDI1) and cell-cycle-related proteins in 12 human liver tumor cell lines (JHH-1, -2, -4, -5, -6, -7; HLE; HuH-7; Hep3B; PLC/PRF/5; HuH-6; HepG2). Seven (JHH-1, -2, -5, -6, -7; Hep3B; HepG2) out of eight cell lines having p21(WAF1/CIP1/SDI1) protein overexpressed cyclin E protein, although one of them (JHH-5) overexpressed a reduced size of cyclin E. The rest (HuH-6) of the 8 cell lines with p21(WAF1/CIP1/SDI1) showed a decreased expression of cyclin E. Four cell lines (JHH-4; HLE; HuH-7; PLC/PRF/5) deficient of p21(WAF1/CIP1/SDI1) protein did not overexpress cyclin E protein. As to expression of the other cell-cycle-related proteins, cyclin A, cyclin D1, CDK2 or CDK4, no significant difference was detected among the 12 cell lines. These findings indicate that the human liver tumor cell lines which have the p21(WAF1/CIP1/SDI1)-inducible barriers of the cell cycle progression can go through the G1/S checkpoint by overexpressing cyclin E.
AB - We examined a relationship between p21(WAF1/CIP1/SDI1) and cell-cycle-related proteins in 12 human liver tumor cell lines (JHH-1, -2, -4, -5, -6, -7; HLE; HuH-7; Hep3B; PLC/PRF/5; HuH-6; HepG2). Seven (JHH-1, -2, -5, -6, -7; Hep3B; HepG2) out of eight cell lines having p21(WAF1/CIP1/SDI1) protein overexpressed cyclin E protein, although one of them (JHH-5) overexpressed a reduced size of cyclin E. The rest (HuH-6) of the 8 cell lines with p21(WAF1/CIP1/SDI1) showed a decreased expression of cyclin E. Four cell lines (JHH-4; HLE; HuH-7; PLC/PRF/5) deficient of p21(WAF1/CIP1/SDI1) protein did not overexpress cyclin E protein. As to expression of the other cell-cycle-related proteins, cyclin A, cyclin D1, CDK2 or CDK4, no significant difference was detected among the 12 cell lines. These findings indicate that the human liver tumor cell lines which have the p21(WAF1/CIP1/SDI1)-inducible barriers of the cell cycle progression can go through the G1/S checkpoint by overexpressing cyclin E.
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U2 - 10.1006/bbrc.1997.7958
DO - 10.1006/bbrc.1997.7958
M3 - Article
C2 - 9446792
AN - SCOPUS:0032515316
SN - 0006-291X
VL - 242
SP - 317
EP - 321
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -