Cyclophilins a and b oppositely regulate renal tubular epithelial cell phenotype

Eduard Sarró*, Mónica Durán, Ana Rico, Diana Bou-Teen, Vanesa Fernández-Majada, Anthony J. Croatt, Karl A. Nath, Maria Teresa Salcedo, Justin H. Gundelach, Daniel Batlle, Richard J. Bram, Anna Meseguer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Restoration of kidney tubular epitheliumfollowing sublethal injury sequentially involves partial epithelial-mesenchymal transition (pEMT), proliferation, and further redifferentiation into specialized tubule epithelial cells (TECs). Because the immunosuppressant cyclosporine-A produces pEMT in TECs and inhibits the peptidyl-prolyl isomerase (PPIase) activity of cyclophilin (Cyp) proteins, we hypothesized that cyclophilins could regulate TEC phenotype. Here we demonstrate that in cultured TECs, CypA silencing triggers loss of epithelial features and enhances transforming growth factor β (TGFβ)-induced EMT in association with upregulation of epithelial repressors Slug and Snail. This pro-epithelial action of CypA relies on its PPIase activity. By contrast, CypB emerges as an epithelial repressor, because CypB silencing promotes epithelial differentiation, prevents TGFβ-induced EMT, and induces tubular structures in 3D cultures. In addition, in the kidneys of CypB knockout mice subjected to unilateral ureteral obstruction, inflammatory and pro-fibrotic events were attenuated. CypB silencing/knockout leads to Slug, but not Snail, downregulation. CypB support of Slug expression depends on its endoplasmic reticulum location, where it interacts with calreticulin, a calciumbuffering chaperone related to Slug expression. As CypB silencing reduces ionomycin-induced calcium release and Slug upregulation, we suggest that Slug expression may rely on CypB modulation of calreticulin-dependent calcium signaling. In conclusion, this work uncovers new roles for CypA and CypB in modulating TEC plasticity and identifies CypB as a druggable target potentially relevant in promoting kidney repair.

Original languageEnglish (US)
Pages (from-to)499-514
Number of pages16
JournalJournal of molecular cell biology
Issue number7
StatePublished - Jul 1 2020


  • Cyclophilins
  • Epithelial phenotype
  • Fibrosis
  • Slug
  • TGFβ
  • UUO

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology


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