Cyclophilins and nucleoporins are required for infection mediated by capsids from circulating HIV-2 primary isolates

João I. Mamede, Florence Damond, Ariel De Bernardo, Sophie Matheron, Diane Descamps, Jean Luc Battini, Marc Sitbon, Valérie Courgnaud*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

HIV-2 groups have emerged from sooty mangabey SIV and entered the human population in Africa on several separate occasions. Compared to world pandemic HIV-1 that arose from the chimpanzee SIVcpz virus, the SIVsm-derived HIV-2, largely confined to West Africa, is less replicative, less transmissible and less pathogenic. Here, we evaluated the interactions between host cellular factors, which control HIV-1 infection and target the capsid, and HIV-2 capsids obtained from primary isolates from patients with different disease progression status. We showed that, like HIV-1, all HIV-2 CA we tested exhibited a dependence on cyclophilin A. However, we observed no correlation between HIV-2 viremia and susceptibility to hu-TRIM5alpha or dependence to CypA. Finally, we found that all CA from HIV-2 primary isolates exploit Nup358 and Nup153 for nucleus transposition. Altogether, these findings indicate that the ability to use the two latter nucleoporins is essential to infection of human cells for both HIV-1 and HIV-2. This dependence provides another molecular target that could be used for antiviral strategies against both HIV-1 and 2, based on both nucleoporins.

Original languageEnglish (US)
Article number45214
JournalScientific reports
Volume7
DOIs
StatePublished - Mar 27 2017

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Cyclophilins and nucleoporins are required for infection mediated by capsids from circulating HIV-2 primary isolates'. Together they form a unique fingerprint.

Cite this