Cyclosporin a stimulates transcription and procollagen secretion in tubulointerstitial fibroblasts and proximal tubular cells

Gunter Wolf, Paul D. Killen, Eric G. Neilson*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

The brief study described in this report was undertaken to determine whether cyclosporin A had any direct effect on the expression of tubulointerstitial procollagens in cultured renal cells. Our findings indicate that murine tubulointerstitial fibroblasts secreted significantly more procollagen type I after the addition of cyclosporin A, whereas syngeneic proximal tubular cells expressed significantly more types I and IV procollagen after cyclosporin stimulation. These increases in procollagen gene product correlated concordantly with changes in the levels of cytoplasmic mRNA with procollagen-specific cDNA probes. Transfection of these fibroblasts and proximal tubular cells with chimeric gene constructs con-taining enhancer/promoter elements for α2(I) and α1(IV) procollagen linked to a chloramphenicol acetyltransferase reporter gene indicates that the stimulatory effect of cyclosporin on procollagen expression depends, at least to some extent, on an increase in transcriptional activity.

Original languageEnglish (US)
Pages (from-to)918-922
Number of pages5
JournalJournal of the American Society of Nephrology
Volume1
Issue number6
StatePublished - Dec 1990

Keywords

  • Cyclosporin A
  • Interstitial fibrosis
  • Nephrotoxicity
  • Procollagen type I
  • Procollagen type IV

ASJC Scopus subject areas

  • Nephrology

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