Cyclosporine a inhibits TNF production without decreasing TNF mRNA levels

Daniel G. Remick*, Dung T. Nguyen, Mark K. Eskandari, Robert M. Strieter, Steven L. Kunkel

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

50 Scopus citations


The role of cytokines in health and disease has received increasing attention and numberous investigations have explored the regulation of cytokine gene expression. Tumor necrosis factor-α (TNF) has received particular attention because of its central role in septic shock and more recent work has shown its participation in transplant immunology. We explored the mechanism of cyclosporine A (CsA) modulation of complete Freunds adjuvant macrophage (CFA-MO) TNF gene expression. From 0.001 to 1 ug/ml, CsA dose-dependently inhibited lipopolysaccharide (LPS) induced secreted bioactivity; at doses above 10μg/ml CSA was directly toxic to CFA-MO. However, there was no suppression of TNF mRNA levels, and CsA also did not inhibit the accumulation of cell-associated TNF. Thus, CsA modulates TNF gene expression in a previously undescribed manner.

Original languageEnglish (US)
Pages (from-to)551-555
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - Jun 15 1989

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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