Cytochrome c oxidase subunit VIIa liver isoform: Characterization and identification of promoter elements in the bovine gene

R. Sathiagana Seelan, Lekha Gopalakrishnan, Richard C. Scarpulla, Lawrence I. Grossman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Cytochrome c oxidase subunit Vila is specified by two nuclear genes, one (COX7AH) producing a heart/musclespecific isoform and the other (COX7AL) a form expressed in all tissues. We have isolated both genes to examine their transcriptional regulation. Here, we characterize the core promoter of COX7AL and show that a 92-base pair region flanking the 5′-end promotes most of the activity of this gene. The 92-bp basal promoter contains sites for the nuclear respiratory factors NRF-1 and NRF-2, which have been shown to contribute to the transcription of a number of nuclear genes involved in mitochondrial respiratory activity, and also at least four Sp1 motifs. We show that both the NRF-1 and NRF-2 binding sites are functional in COX7AL and present evidence suggesting that interaction between the NRF-1 site and an upstream element contributes to expression.

Original languageEnglish (US)
Pages (from-to)2112-2120
Number of pages9
JournalJournal of Biological Chemistry
Volume271
Issue number4
DOIs
StatePublished - Jan 26 1996

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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