TY - JOUR
T1 - Cytochrome c oxidase subunit VIIa liver isoform
T2 - Characterization and identification of promoter elements in the bovine gene
AU - Seelan, R. Sathiagana
AU - Gopalakrishnan, Lekha
AU - Scarpulla, Richard C.
AU - Grossman, Lawrence I.
PY - 1996/1/26
Y1 - 1996/1/26
N2 - Cytochrome c oxidase subunit Vila is specified by two nuclear genes, one (COX7AH) producing a heart/musclespecific isoform and the other (COX7AL) a form expressed in all tissues. We have isolated both genes to examine their transcriptional regulation. Here, we characterize the core promoter of COX7AL and show that a 92-base pair region flanking the 5′-end promotes most of the activity of this gene. The 92-bp basal promoter contains sites for the nuclear respiratory factors NRF-1 and NRF-2, which have been shown to contribute to the transcription of a number of nuclear genes involved in mitochondrial respiratory activity, and also at least four Sp1 motifs. We show that both the NRF-1 and NRF-2 binding sites are functional in COX7AL and present evidence suggesting that interaction between the NRF-1 site and an upstream element contributes to expression.
AB - Cytochrome c oxidase subunit Vila is specified by two nuclear genes, one (COX7AH) producing a heart/musclespecific isoform and the other (COX7AL) a form expressed in all tissues. We have isolated both genes to examine their transcriptional regulation. Here, we characterize the core promoter of COX7AL and show that a 92-base pair region flanking the 5′-end promotes most of the activity of this gene. The 92-bp basal promoter contains sites for the nuclear respiratory factors NRF-1 and NRF-2, which have been shown to contribute to the transcription of a number of nuclear genes involved in mitochondrial respiratory activity, and also at least four Sp1 motifs. We show that both the NRF-1 and NRF-2 binding sites are functional in COX7AL and present evidence suggesting that interaction between the NRF-1 site and an upstream element contributes to expression.
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U2 - 10.1074/jbc.271.4.2112
DO - 10.1074/jbc.271.4.2112
M3 - Article
C2 - 8567667
AN - SCOPUS:0030022934
SN - 0021-9258
VL - 271
SP - 2112
EP - 2120
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 4
ER -