Cytochrome c peroxidase - Cytochrome c complex: Locating the second binding domain on cytochrome c peroxidase with site-directed mutagenesis

V. W. Leesch*, J. Bujons, A. G. Mauk, B. M. Hoffman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

Cytochrome c peroxidase (CcP) can bind as many as two cytochrome c (Cc) molecules in an electrostatic complex. The location of the two binding domains on CcP has been probed by photoinduced interprotein electron transfer (ET) between zinc-substituted horse cytocthrome c (ZnCc) and CcP with surface charge-reversal mutations and by isothermal titration calorimetry (ITC). These results, which are the first experimental evidence for the location of domain 2, indicate that the weak-binding domain includes residues 146-150 on CcP. CcP(E290K) has a charge-reversal mutation in the tight-binding domain, which should weaken binding, and it weakens the 1:1 complex; K1 decreases 20-fold at 18 mM ionic strength. We have employed two mutations to probe the proposed location for the weak-binding domain on the CcP surface: (i) D148K, a 'detrimental' mutation with a net (+2) change in the charge of CcP, and (ii) K149E, a 'beneficial' mutation with a net (-2) change in the charge. The interactions between FeCc and CcP(WT and K149E) also have been studied with ITC. The CcP(D148K) mutation causes no substantial change in the 2:1 binding but an increase in the reactivity of the 2:1 complex. The latter can be interpreted as a long-range influence on the heme environment or, more likely, the enhancement of a minority subset of binding conformations with favorable pathways for ET. CcP(K149E) has a charge-reversal mutation in the weak-binding domain that produces a substantial increase in the 2:1 binding constant as measured by both quenching and ITC. For the 1:1 complex of CcP(WT), ΔG1 = -8.2 kcal/mol (K1 = 1.3 x 106 M-1), ΔH1 = +2.7 kcal/mol, and ΔS1 = +37 cal/K·mol at 293 K; for the second binding stage, K2 < 5 x 103 M-1, but accurate thermodynamic parameters were not obtained. For the 1:1 complex of CcP-(K149E), ΔG1 = -8.5 kcal/mol (K1 = 2 x 106 M-1), ΔH1 = +2.0 kcal/mol, and ΔS1 = +36 cal/K·mol; for the second stage, ΔG2 = -5.5 kcal/mol (K1 = 1.3 x 104 M-1), ΔH2 = +2.9 kcal/mol, and ΔS2 = +29 cal/K·mol.

Original languageEnglish (US)
Pages (from-to)10132-10139
Number of pages8
JournalBiochemistry
Volume39
Issue number33
DOIs
StatePublished - Aug 22 2000

ASJC Scopus subject areas

  • Biochemistry

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